Newly proliferated cells in the adult male amygdala are affected by gonadal steroid hormones
Newly proliferated cells in the adult male amygdala are affected by gonadal steroid hormones
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DOI：
10.1002/neu.10273
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发表时间：
2003-12-01
期刊：
影响因子：
--
通讯作者：
Wang, ZX
中科院分区：
文献类型：
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作者：
Fowler, CD;Freeman, ME;Wang, ZX
性腺类固醇激素在神经元的增殖、存活和激活中起重要作用。本研究旨在探讨睾酮及其代谢产物对成年雄性北美田鼠（Microtus pennaculicus）杏仁核新增殖细胞的影响。丙酸睾酮（TP）治疗去势雄性导致血浆睾酮水平与交配后的雄性相似。TP处理的雄性动物杏仁核中细胞增殖标记物（BrdU）标记的细胞密度显著增加。苯甲酸雌二醇（EB）治疗产生了类似的效果TP的BrdU标记的细胞的密度，而5 α-双氢睾酮（DHT）是无效的。杏仁核中较大比例（约44%）的BrdU标记细胞显示神经元表型，较小比例（约35%）显示胶质祖细胞表型;然而，在两种细胞群体中均未发现治疗效应。激素效应似乎具有部位特异性，因为在海马齿状回或腹内侧下丘脑中未发现组间差异。最后，一项时间进程研究表明，早在急性注射BrdU后30分钟，杏仁核中就存在BrdU标记的细胞。总之，这些数据表明，性腺类固醇激素影响杏仁核中新增殖细胞的数量，最有可能通过雌激素机制发挥作用，这些作用可能会施加在杏仁核内的局部增殖祖细胞。(C)2003 Wiley Periodicals，Inc.
Gonadal steroid hormones play an important role in the proliferation, survival, and activation of neurons. The present study was performed to examine the effects of testosterone and its metabolites on newly proliferated cells in the amygdala of adult male meadow voles (Microtus pennsylvanicus). Treatment with testosterone propionate (TP) in castrated males resulted in plasma testosterone levels similar to males following mating. TP-treated males displayed a significant increase in the density of cells labeled with a cell proliferation marker (BrdU) in the amygdala. Treatment with estradiol benzoate (EB) exerted a similar effect as TP on the density of BrdU-labeled cells, whereas 5alpha-dihydrotestosterone (DHT) was ineffective. A larger proportion (approximate to44%) of the BrdU-labeled cells in the amygdala displayed a neuronal phenotype, and a lesser percentage (approximate to35%) displayed a glial progenitor phenotype; however, treatment effects were not found in either population of cells. Hormonal effects appeared to be site-specific as no group differences were found in the dentate gyrus of the hippocampus or ventromedial hypothalamus. Finally, a time course study indicated that BrdU-Iabeled cells in the amygdala are present as early as 30 min following an acute injection of BrdU. Together, these data suggest that gonadal steroid hormones influence the number of newly proliferated cells in the amygdala, most likely by acting through an estrogenic mechanism, and these effects may be exerted on locally proliferating progenitors within the amygdala. (C) 2003 Wiley Periodicals, Inc.