Intron and intronless transcription of the chicken polyubiquitin gene UbII
Intron and intronless transcription of the chicken polyubiquitin gene UbII
复制标题
DOI：
10.1016/0014-5793(93)80555-9
复制
发表时间：
1993-03
期刊：
影响因子：
3.5
通讯作者：
J. Mezquita;B. López-Ibor;M. Pau;C. Mezquita
中科院分区：
文献类型：
--
作者：
J. Mezquita;B. López-Ibor;M. Pau;C. Mezquita
我们以前曾报道，鸡多聚泛素基因UbII的精子发生过程中优先表达，我们在这里显示，UbII是主要的多聚泛素基因在早期胚胎发生中表达。检测到两个主要的起始位点。从早期胚胎中使用的起始位点转录导致转录物的5 '-非翻译区中存在内含子，如已报道的其他聚泛素信息。然而，在成熟睾丸中，使用位于内含子内的不同起始位点产生无内含子转录本。不同的启动子序列，存在于每个起始位点，可以调节在该基因中观察到的差异表达。
We have previously reported that the chicken polyubiquitin gene UbII is preferentially expressed during spermatogenesis and we show here that UbII is the predominant polyubiquitin gene expressed in early embryogenesis. Two main initiation sites were detected. Transcription from the initiation site used in early embryos results in the presence of an intron in the 5'-untranslated region of the transcripts as has been reported for other polyubiquitin messages. In mature testis, however, the use of a different initiation site, located within the intron, produces intronless transcripts. Distinct promoter sequences, present in each initiation site, may regulate the differential expression observed in this gene.