THE POLY(A)(+)RNA SEQUENCE COMPLEXITY IS ALSO REPRESENTED IN POLY(A)(-)RNA IN SEA-URCHIN EMBRYOS
THE POLY(A)(+)RNA SEQUENCE COMPLEXITY IS ALSO REPRESENTED IN POLY(A)(-)RNA IN SEA-URCHIN EMBRYOS
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DOI：
10.1111/j.1432-0436.1984.tb00262.x
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发表时间：
1984-01-01
期刊：
影响因子：
2.9
通讯作者：
HUMPHREYS, T
中科院分区：
文献类型：
--
作者：
DUNCAN, R;HUMPHREYS, T
通过c[互补]DNA交叉杂交，确定了海胆胚胎的poly（A）（+）RNA序列复杂性在多大程度上也表现为poly（A）（-）RNA。细胞质poly（A）（+）RNA和多聚体poly（A）（+）RNA序列中80%或更多以poly（A）（-）形式出现。在这两种情况下，与cDNA反应的poly（A）（-）RNA分子的细胞浓度与同源poly（A）（+）序列的浓度相似。几乎没有（如果有的话）丰富的poly（A）（+）mRNA分子被聚腺苷酸化定量区分，因为丰富的poly（A）（+）序列在poly（A）（-）RNA中也很丰富。无论是降解或低效率的结合寡（dT）-纤维素可以解释所观察到的交叉反应性。在海胆胚胎中，poly（A）显然不通过划分mRNA亚群来调节mRNA的利用，所述mRNA亚群是特异性且完全聚腺苷酸化的。
The extent to which the poly(A)(+)RNA sequence complexity from sea-urchin embryos is also represented in poly(A)(-)RNA was determined by c[complementary]DNA cross-hybridization. Eighty percent or more of both the cytoplasmic poly(A)(+)RNA and polysomal poly(A)(+)RNA sequences appeared in a poly(A)(-) form. In both cases, the cellular concentrations of the poly(A)(-)RNA molecules that reacted with the cDNA were similar to the concentrations of the homologous poly(A)(+) sequences. Few, if any, abundant poly(A)(+)mRNA molecules were quantitatively discriminated by polyadenylation, since the abundant poly(A)(+) sequences were also abundant in poly(A)(-)RNA. Neither degradation nor inefficient binding to oligo (dT)-cellulose can account for the observed cross-reactivity. In sea-urchin embyros, the poly(A) evidently does not regulate utilization of mRNA by demarcating an mRNA subset is specifically and completely polyadenylated.