A novel polysaccharide from the seeds of Plantago asiatica L. induces dendritic cells maturation through toll-like receptor 4
A novel polysaccharide from the seeds of Plantago asiatica L. induces dendritic cells maturation through toll-like receptor 4
复制标题
来自车前子种子的新型多糖通过 Toll 样受体 4 诱导树突状细胞成熟
DOI：
10.1016/j.intimp.2013.11.024
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发表时间：
2014-02-01
影响因子：
5.6
通讯作者：
Xie, Mingyong
中科院分区：
文献类型：
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作者：
Huang, Danfei;Nie, Shaoping;Xie, Mingyong
本研究研究了车前草种子纯化多糖（PLP-2）对小鼠骨髓源性树突状细胞（DCs）表型和功能成熟的影响及其机制。结果显示，PLP-2增加了成熟标志物主要组织相容性复合体II、CD86、CD80和CD40在dc上的表达。与表型标记的变化一致，dc成熟的功能分析显示，PLP-2减少了dc的内吞作用，增加了细胞内白细胞介素(IL)-12水平和异源刺激活性。此外，利用同源T细胞活化模型，我们发现PLP-2处理的树突状细胞更有效地向T细胞提供卵清蛋白抗原，这可以通过增加T细胞增殖来证明。此外，在PLP-2处理之前，用抗TLR4抗体处理细胞，PLP-2对DCs的作用显著减弱，这表明PLP-2和TLR4在细胞表面直接相互作用。这些结果表明PLP-2可能通过TLR4诱导DCs成熟。本研究结果对了解植物多糖免疫增强作用的分子机制具有重要意义。(C) 2013 Elsevier B.V.版权所有
In this study, we investigated the effect of a polysaccharide purified from the seeds of Plantago asiatica L (PLP-2) on the phenotypic and functional maturation of murine bone marrow-derived dendritic cells (DCs) and relevant mechanisms. The results showed that PLP-2 increased the expression of maturation markers major histocompatibility complex II, CD86, CD80, and CD40 on DCs. Consistent with the changes in the phenotypic markers, functional assay for DCs maturation showed that PLP-2 decreased DCs endocytosis and increased intracellular interleukin (IL)-12 levels and allostimulatory activity. Furthermore, using a syngeneic T cell activation model, we found that PLP-2 treated DCs presented ovalbumin antigen to T cells more efficiently as demonstrated by increased T cell proliferation. In addition, the effects of PLP-2 on DCs were significantly impaired by treating the cells with anti-TLR4 antibody prior to PLP-2 treatment, implying direct interaction between PLP-2 and TLR4 on cell surface. These results suggested that PLP-2 may induce DCs maturation through TLR4. Our results may have important implications for our understanding on the molecular mechanisms of immunopotentiating action of the polysaccharides from plants. (C) 2013 Elsevier B.V. All rights reserved.