Diversity in cell specific co-expression of four neuropeptide genes involved in control of male copulation behaviour in Lymnaea stagnalis
Diversity in cell specific co-expression of four neuropeptide genes involved in control of male copulation behaviour in Lymnaea stagnalis
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DOI：
10.1016/s0306-4522(96)00576-3
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发表时间：
1997-05-01
期刊：
影响因子：
3.3
通讯作者：
VanMinnen, J
中科院分区：
文献类型：
--
作者：
DeLange, RPJ;VanGolen, FA;VanMinnen, J
我们在此报告了编码参与控制男性行为的神经肽的四种基因的神经元特异性共表达。这些神经元位于 Lymnaea stagnalis 中枢神经系统的右脑神经节前叶，并通过阴茎神经投射到阴茎复合体。为了实现最佳保证，我们应用了原位杂交、免疫细胞化学和基质辅助激光解吸电离质谱。前叶神经元表达编码酰胺化四肽 APGWamide 的基因。现在显示这些细胞的亚群专门与其他三种神经肽基因之一共表达 APGWamide 基因，分别编码 Lymnaea 神经肽 Y、conopressin 或踏板肽。所有四个基因也在投射到阴茎复合体的其他中心的其他神经元中表达，但在这些细胞中没有观察到共表达。基于免疫细胞化学和质谱分析，可以在前叶细胞体中鉴定出由基因编码的神经肽。神经肽 APGWamide 和 Lymnaea 神经肽 Y 共定位于前叶细胞以及支配阴茎牵开肌的轴突中，不会诱导肌肉收缩，但通过影响收缩的舒张率和幅度而具有调节作用。 APGWamide 和 conopressin 早些时候被认为可以调节输精管的蠕动。因此，前叶中共同表达神经肽基因的各种组合的神经元似乎代表了功能单元，每个单元都在微调涉及男性交配行为特定方面的不同肌肉。 (C) 1997 国际广播组织。
We report here the neuron-specific co-expression of four genes coding for neuropeptides involved in the control of male behaviour. These neurons are located in the anterior lobe of the right cerebral ganglion in the central nervous system of Lymnaea stagnalis and project via the penis nerve to the penial complex. In order to accomplish optimal assurance we applied in situ hybridization, immunocytochemistry and matrix-assisted laser desorption ionization mass spectrometry. The anterior lobe neurons express the gene encoding the amidated tetrapeptide APGWamide. Subsets of these cells are now shown to co-express the APGWamide gene exclusively with one of three other neuropeptide genes, encoding Lymnaea neuropeptide Y, conopressin or pedal peptide, respectively. All four genes are also expressed in other neurons in other centres projecting to the penial complex, but in these cells co-expression was not observed. The neuropeptides encoded by the genes could be identified in the anterior lobe cell bodies on the basis of immunocytochemistry and mass spectrometrical analysis. The neuropeptides APGWamide and Lymnaea neuropeptide Y, which are co-localized in the anterior lobe cells as well as in axons innervating the penis retractor muscle, do not induce muscle contraction but have a modulatory action by affecting the relaxation rate and amplitude of the contraction. APGWamide and conopressin had earlier been suggested to modulate peristalsis of the vas deferens.Thus, it seems that the neurons co-expressing the various combinations of neuropeptide genes in the anterior lobe represent functional units, each acting in the fine tuning of different muscles involved in specific aspects of male copulation behaviour. (C) 1997 IBRO.