Structure of late summer phytoplankton community in the Firth of Lorn (Scotland) using microscopy and HPLC-CHEMTAX
Structure of late summer phytoplankton community in the Firth of Lorn (Scotland) using microscopy and HPLC-CHEMTAX
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DOI：
10.1016/j.ecss.2015.07.006
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发表时间：
2015-12
影响因子：
2.8
通讯作者：
A. Brito;C. Sá;C. R. Mendes;T. Brand;A. Dias;V. Brotas;K. Davidson
中科院分区：
文献类型：
--
作者：
A. Brito;C. Sá;C. R. Mendes;T. Brand;A. Dias;V. Brotas;K. Davidson
洛恩湾位于苏格兰最大的峡湾之一的林赫湖的入海口。这个海湖由许多其他内湖喂养，提供了大量的淡水，这经常导致水柱分层。为了研究环境条件如何影响该地区浮游植物的空间分布，我们收集了水样（浮游植物色素和显微镜）以及其他环境变量（包括营养成分）。利用化学分类学方法估算了不同分类类群对浮游植物生物量指数的贡献。化学分类与显微数据吻合良好。在奥班湾附近，绿藻含量最高（~ 2.6 mg m−3），在分层水体中，最丰富的类群隐生植物、鞭毛藻和其他鞭毛藻大量繁殖。中心硅藻，主要是毛藻。与nh4和sio2浓度和分层有关，而pennate硅藻，主要是圆柱形硅藻。andNitzchiasp。，与NO3+ no2和高表面混合层深度有关。根据它们的表面体积比以及它们对环境参数（营养物质）和湍流的亲和力，确定了四个硅藻群。本研究采用物理化学数据、经典显微镜方法（适用于bbb20 μm大细胞）和HPLC-CHEMTAX方法（适用于大细胞和小细胞）相结合的方法，对峡湾沿岸地区浮游植物功能群的分布进行了评价。
The Firth of Lorn is at the mouth of one of Scotland's largest fjordic sea lochs, Loch Linnhe. This sea loch, which is fed by a number of other inner lochs, supplies a significant flow of freshwater, which frequently causes the stratification of the water column. To investigate how environmental conditions influence the spatial distribution of phytoplankton in this region water samples were collected for phytoplankton (pigments and microscopy), and other environmental variables including nutrients. Chemotaxonomy was used to estimate the contribution of different taxonomic groups to total chlorophylla(phytoplankton biomass index). Good agreement was obtained between chemotaxonomy and microscopy data. The highest levels of chlorophylla(∼2.6 mg m−3) were found in the vicinity of Oban Bay, where cryptophytes, the most abundant group, dinoflagellates and other flagellates thrived in the stratified water column. Centric diatoms, mainlyChaetocerossp. andSkeletonema costatum, were associated with NH4and SiO2concentrations and stratification, while pennate diatoms, mainlyCylindrothecasp. andNitzchiasp., were found to be associated with NO3+ NO2and high surface mixed layer depths. Four diatom groups were identified in accordance to their surface to volume ratios, as well as their affinity to environmental parameters (nutrients) and turbulence. This study used a combination of physico-chemical data, classical microscopy methods (appropriate for large cells > 20 μm) and HPLC-CHEMTAX approaches (for large and small cells) to evaluate the distribution of phytoplankton functional groups in a fjordic coastal area.