Tumor collagenase stimulatory factor (TCSF) expression and localization in human lung and breast cancers
Tumor collagenase stimulatory factor (TCSF) expression and localization in human lung and breast cancers
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DOI：
10.1177/002215549704500508
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发表时间：
1997-05-01
影响因子：
3.2
通讯作者：
Birembaut, P
中科院分区：
文献类型：
--
作者：
Polette, M;Gilles, C;Birembaut, P
肿瘤细胞源性胶原酶刺激因子 (TCSF) 在体外刺激参与肿瘤侵袭的各种基质金属蛋白酶的生物合成，例如间质胶原酶、明胶酶 A 和溶基质素 1。使用原位杂交和 Northern 印迹分析，在 7 种正常组织和 22 例肺鳞状细胞癌以及 7 例良性增殖和肺癌中研究了 TCSF mRNA 的体内表达。 22 例乳腺导管癌。通过原位杂交，在 44 种癌症中的 40 种（肺癌和乳腺癌的浸润前和浸润癌细胞中）检测到了 TCSF mRNA。 TCSF mRNA 和明胶酶 A mRNA 均在乳腺癌连续切片的相同区域中可见，并由不同的细胞、肿瘤细胞和成纤维细胞表达。组织学结果通过 Northern blot 分析得到证实，结果显示癌症中 TCSF mRNA 的表达高于良性和正常组织。这些观察结果支持以下假设：TCSF 是肺癌和乳腺肿瘤进展的重要因素。
Tumor cell-derived collagenase stimulatory factor (TCSF) stimulates in vitro the biosynthesis of various matrix metalloproteinases involved in tumor invasion, such as interstitial collagenase, gelatinase A, and stromelysin 1. The expression of TCSF mRNAs was studied in vivo, using in situ hybridization and Northern blotting analysis, in seven normal tissues and in 22 squamous cell carcinomas of the lung, and in seven benign proliferations and in 22 ductal carcinomas of the mammary gland. By in situ hybridization, TCSF mRNAs were detected in 40 of 44 carcinomas, in pre-invasive and invasive cancer cells of both lung and breast cancers. TCSF mRNAs and gelatinase A mRNAs were both visualized in the same areas in serial sections in breast cancers, and were expressed by different cells, tumor cells, and fibroblasts. The histological results were confirmed by Northern blot analysis, which showed a higher expression of TCSF mRNAs in cancers than in benign and normal tissues. These observations support the hypothesis that TCSF is an important factor in lung and breast tumor progression.