NUCLEOTIDE-SEQUENCE OF THE LIG GENE AND PRIMARY STRUCTURE OF DNA-LIGASE OF ESCHERICHIA-COLI
NUCLEOTIDE-SEQUENCE OF THE LIG GENE AND PRIMARY STRUCTURE OF DNA-LIGASE OF ESCHERICHIA-COLI
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DOI：
10.1007/bf00330179
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发表时间：
1986-01-01
期刊：
影响因子：
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通讯作者：
NAKATA, A
中科院分区：
文献类型：
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作者：
ISHINO, Y;SHINAGAWA, H;NAKATA, A
大肠杆菌的DNA连接酶催化DNA中5“-磷酰基和3”-羟基之间的磷酸二酯键的NAD依赖性形成。它对DNA复制和修复受损的DNA链至关重要。我们测定了大肠杆菌编码DNA连接酶的lig基因及其侧翼区的核苷酸序列。该基因的编码框架被证实的氨基酸组成和纯化的连接酶的氨基和羧基末端的氨基酸序列。该连接酶由671个氨基酸残基组成，分子量为73，690。
The DNA ligase of Escherichia coli catalyses the NAD-dependent formation of phosphodiester linkages between 5''-phosphoryl and 3''-hydroxyl groups in DNA. It is essential for DNA replication and repair of damaged DNA strands. We determined the nucleotide sequence of the lig gene of Escherichia coli coding for DNA ligase and flanking regions. The coding frame of the gene was confirmed by the amino acid composition and the amino- and carboxyl-terminal amino acid sequences of the purified ligase. The ligase consists of 671 amino acid residues with a molecular weight of 73,690.