3D-reconstruction of blood vessels by ultramicroscopy
3D-reconstruction of blood vessels by ultramicroscopy
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DOI：
10.4161/org.5.4.10403
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发表时间：
2009-10-01
期刊：
影响因子：
2.3
通讯作者：
Dodt, Hans-Ulrich
中科院分区：
文献类型：
--
作者：
Jaehrling, Nina;Becker, Klaus;Dodt, Hans-Ulrich
正如最近所显示的，超微技术(UM)能够以微米分辨率对大型微观结构进行3D可视化。因此，它可以应用于大量生物和医学标本的解剖学研究。我们用UM技术重建了番茄凝集素(Lycopersicesculentum)染色的小鼠器官血管网络的三维结构。微血管丝状分支的拓扑图可见。由于肿瘤需要广泛的血管生长才能存活，这种新的方法可能会在神经生物学和组织学方面开辟新的前景，特别是在癌症研究方面。
As recently shown, ultramicroscopy (UM) allows 3D-visualization of even large microscopic structures with mu m resolution. Thus, it can be applied to anatomical studies of numerous biological and medical specimens. We reconstructed the three-dimensional architecture of tomato-lectin (Lycopersicon esculentum) stained vascular networks by UM in whole mouse organs. The topology of filigree branches of the microvasculature was visualized. Since tumors require an extensive growth of blood vessels to survive, this novel approach may open up new vistas in neurobiology and histology, particularly in cancer research.