Alterations in T2 relaxation magnetic resonance imaging of the ovine intervertebral disc due to nonenzymatic glycation.
Alterations in T2 relaxation magnetic resonance imaging of the ovine intervertebral disc due to nonenzymatic glycation.
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DOI：
10.1097/brs.0b013e31822ce81f
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发表时间：
2012-02-15
期刊：
影响因子：
3
通讯作者：
Tang SY
中科院分区：
文献类型：
--
作者：
Jazini E;Sharan AD;Morse LJ;Dyke JP;Aronowitz EB;Chen LK;Tang SY
一项使用羊椎间盘的体外研究，旨在通过磁共振成像（MRI）评估晚期糖基化终产物（AGEs）与椎间盘水合作用的相关性。目的：应用T2弛豫MRI研究椎间盘组织中AGEs含量与组织含水量的关系。AGEs由非酶糖化产生，并且AGEs已显示随着老化和变性在IVD组织中积累。AGEs可以改变生物化学性质，包括细胞外基质的疏水性。由于IVD的退行性体征之一是水合作用减少，因此假设组织AGEs水平升高可能导致椎间盘水合作用。T2弛豫MRI已被证明对椎间盘的水合状态敏感，并且在检测由AGEs增加介导的IVD变化方面可能有价值。从4个绵羊脊柱中获得38个IVD，并从这些椎间盘中分离纤维环（AF）和髓核（NP）组织。将组织在核糖基化或对照溶液中孵育长达8天以诱导AGEs的形成。随后分析这些组织的组织含水量和AGEs浓度。T2弛豫时间从核糖基化后的这些组织中获得。核糖基化导致AF和NP中AGEs的积累增加，并且以剂量依赖性方式降低含水量。当通过MRI分析时，核糖基化显著改变了NP中的平均T2弛豫时间（p=0.001），但在AF中没有（p=0.912）。此外，随着孵育时间的增加，NP中的平均T2值显著降低（p<0.001）。这项研究表明，在IVD的AGEs水平可能会影响组织含水量。此外，这些核糖基化介导的组织水合作用的变化可使用T2弛豫MRI检测。T2弛豫MRI可以提供一种非侵入性的工具来测量与AGEs积累呈负相关的椎间盘水合作用的体内变化。
An in vitro study using ovine intervertebral discs to correlate the effects of advanced glycation end-products (AGEs) with disc hydration evaluated by magnetic resonance imaging (MRI). To determine the relationship between the level of AGEs and tissue water content in intervertebral discs using T2 relaxation MRI. AGEs result from nonenzymatic glycation, and AGEs have been shown to accumulate in the IVD tissue with aging and degeneration. AGEs can alter biochemical properties, including the hydrophobicity of the extracellular matrix. Since one of the degenerative signs of the IVD is the reduced hydration, it was hypothesized that increased levels of tissue AGEs may contribute to disc hydration. T2 relaxation MRI has been shown to be sensitive to the hydration status of the disc, and may be valuable in detecting the changes in the IVD mediated by the increase of AGEs. Thirty-eight IVDs were obtained from 4 ovine spines, and the annulus fibrosis (AF) and nucleus pulposus (NP) tissues were isolated from these discs. The tissues were incubated in either a ribosylation or control solution for up to 8 days to induce the formation of AGEs. These tissues were subsequently analyzed for tissue water content and concentration of AGEs. T2 relaxation times were obtained from these tissues after ribosylation. Ribosylation led to the increased accumulation of AGEs and reduced water content in both the AF and NP in a dose-dependent manner. When analyzed by MRI, ribosylation significantly altered the mean T2 relaxation times in the NP (p=0.001), but not in the AF (p=0.912). Furthermore, the mean T2 values in the NP significantly decreased with increasing periods of incubation time (p<0.001). This study demonstrates that levels of AGEs in the IVD may affect the tissue water content. Moreover, these ribosylation-mediated changes in tissue hydration were detectable using T2 relaxation MRI. T2 relaxation MRI may provide a non-invasive tool to measure in vivo changes in disc hydration that are negatively correlated with the accumulation of AGEs.