Subcellular localization of chitinase and β-1,3-glucanase in compatible and incompatible interactions between wheat and Puccinia striiformis f. sp. tritici
Subcellular localization of chitinase and β-1,3-glucanase in compatible and incompatible interactions between wheat and Puccinia striiformis f. sp. tritici
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发表时间：
2003
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通讯作者：
Z. Kang;Lili Huang;H. Buchenauer
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作者：
Z. Kang;Lili Huang;H. Buchenauer
利用烟草酸性几丁质酶和酸性β-1，3-葡聚糖酶的抗血清，研究了这两种酶在小麦与条锈菌（Pucciniastriiformis f.）利用免疫金标记技术，对三疣梭子蟹sp.研究表明，两种酶的标记模式在未接种和感染的小麦叶片中非常相似。酶主要定位于宿主细胞壁，而在细胞质和细胞器中没有观察到标记。但在感病和抗病小麦叶片中几丁质酶和β-1，3-葡聚糖酶的积累量存在显著差异。在感病品种的感病叶片中，这两种酶的标记密度。与未接种的对照相比，“壶仙红”的几丁质酶和β-1，3-葡聚糖酶的标记密度略有增加，而抗病品种的几丁质酶和β-1，3-葡聚糖酶的标记密度显著高于未接种的对照。‘西农85’与未接种叶片比较。此外，几丁质酶和β-1，3-葡聚糖酶也在小麦叶片的吸器外基质和真菌细胞壁上发生标记。抗病品种感病叶片中的吸器外基质和菌丝细胞壁。“西农85”的标记密度通常高于感病品种。胡先红。此外，在抗病小麦品种的侵染叶片中，这两种酶也在细胞壁的附着处被检测到。几丁质酶和β-1，3-葡聚糖酶积累在小麦不亲和互作防御反应中的潜在作用。'西农85'和小麦条锈菌P. striiformis f. sp. triangulae进行了讨论。
Two antisera raised against acidic chitinase and acidic β-1,3-glucanase from tobacco were used to investigate the subcellular localization of the two enzymes in the compatible and incompatible interactions between wheat and Puccinia striiformis f. sp. tritici by means of the immunogold labelling technique. The studies demonstrated that the labelling patterns for both enzymes were very similar in the uninoculated and infected wheat leaves. The enzymes were localized mainly in the host cell walls, while no labelling was observed in cytoplasm and organelles. However, the accumulation of chitinase and β-1,3-glucanase in the infected wheat leaves differed markedly between resistant and susceptible wheat cultivars. The labelling densities for the two enzymes in the infected leaves of the susceptible cv. 'Huxianhong' increased slightly as compared to the uninoculated leaves, whereas significantly higher labelling densities of chitinase and β-1,3-glucanase were found in the infected leaves of the resistant cv. 'Xinong 85' compared to the uninoculated leaves. Furthermore, the labelling of chitinase and β-1,3-glucanase also occurred over the extrahaustorial matrix and the fungal cell walls in the infected wheat leaves. The extrahaustorial matrix and the hyphal cell walls in the infected leaves of the resistant cv. 'Xinong 85' usually showed a higher density of the labelling than those in the susceptible cv. 'Huxianhong'. In addition, the two enzymes were also detected over the cell wall appositions in the infected leaves of the resistant wheat cultivar. The potential role of chitinase and β-1,3-glucanase accumulation in the defense reactions of the incompatible interaction of wheat cv. 'Xinong 85' and P. striiformis f. sp. tritici is discussed.