Nicotianamine Synthase Gene 1 from the hyperaccumulator Sedum alfredii Hance is associated with Cd/Zn tolerance and accumulation in plants
Nicotianamine Synthase Gene 1 from the hyperaccumulator Sedum alfredii Hance is associated with Cd/Zn tolerance and accumulation in plants
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DOI：
10.1007/s11104-019-04233-4
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发表时间：
2019-08
期刊：
影响因子：
4.9
通讯作者：
Shaoning Chen;Min Zhang;Ying Feng;Z. A. Sahito;S. Tian;Xiaoe Yang
中科院分区：
文献类型：
--
作者：
Shaoning Chen;Min Zhang;Ying Feng;Z. A. Sahito;S. Tian;Xiaoe Yang
Background and aimsSedum alfrediiHance is a hyperaccumulator of cadmium (Cd) and zinc (Zn) that exhibits extraordinary accumulation of these metals in various tissues. TheNicotianamine synthase(NAS) genes play key roles in regulating the production of nicotianamine, a non-protein amino acid that facilitates metal homeostasis in plants, but the functions of these genes inS. alfrediiremain unknown. The aims of this study were to identify and characterize theNASgene fromS. alfredii(SaNAS1) and exploreNASroles in Cd or Zn tolerance and accumulation.MethodsOne Cd-inducedNASgene fromS. alfredii(SaNAS1) was cloned. We determined the subcellular localization of the encoded protein and evaluatedSaNAS1expression. We also assessedSaNAS1function by conducting a complementary assay with yeast mutantsΔzrc1exposed to Cd or Zn. Finally, transgenicArabidopsisplants expressingSaNAS1were produced, andSaNAS1function was further examined in these plants.ResultsSaNAS1was highly expressed in response to Cd or Zn exposure, and the encoded protein was distributed throughout the cytoplasm and nucleus. Furthermore, yeast expressingSaNAS1exhibited increased tolerance to Cd or Zn. Finally, expression ofSaNAS1inArabidopsisincreased nicotianamine production and promoted Cd or Zn accumulation in roots and shoots.SaNAS1-expressing transgenicArabidopsislines showed improved seedling growth under Cd or Zn stress compared with wild type, indicating thatSaNAS1enhances tolerance to both Cd and Zn.ConclusionsSaNAS1may play a critical role in Cd or Zn tolerance and hyperaccumulation by regulating nicotianamine level inS. alfredii.
