Human papilloma virus prevalence in laryngeal squamous cell carcinoma
Human papilloma virus prevalence in laryngeal squamous cell carcinoma
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DOI：
10.1017/s0022215107006482
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发表时间：
2007-08-01
影响因子：
1.7
通讯作者：
Dursun, E.
中科院分区：
文献类型：
--
作者：
Gungor, A.;Cincik, H.;Dursun, E.
目的：确定喉鳞状细胞癌病例中人乳头瘤病毒脱氧核糖核酸（DNA）的流行情况和类型。方法：我们采用聚合酶链反应技术分析了1990年至2005年间99例喉鳞状细胞癌存档石蜡块标本中人乳头瘤病毒感染的流行情况。来自五个已证实的疣状皮肤病变的活检标本被用作阳性对照，来自五名健康志愿者的外周血样本被用作阴性对照。 结果：发现四个测试样本的脱氧核糖核酸纯度不足，因此被排除在研究之外。 95例喉鳞状细胞癌中有7例检出人乳头瘤病毒脱氧核糖核酸（7.36%）。人乳头瘤病毒基因分型显示，3 例双重人乳头瘤病毒感染，其余 4 例为单一人乳头瘤病毒感染。检测到的人乳头瘤病毒基因型为6、11和16（后者仅在1例中检出）。结论：在我们的系列研究中，发现喉鳞状细胞癌病例中人乳头瘤病毒的患病率非常低。检测到的人乳头瘤病毒基因型多为6型和/或11型，仅1例为16型。据我们所知，这是基于土耳其人群的聚合酶链反应基因分型，首次报告人乳头状瘤病毒在喉鳞状细胞癌中的流行。
Purpose: To determine the prevalence and type of human papilloma virus deoxyribonucleic acid (DNA) in cases of laryngeal squamous cell carcinoma.Methods: We analysed the prevalence of human papilloma virus infection in archived paraffin block specimens taken from 99 cases of laryngeal squamous cell carcinoma between 1990 and 2005, using polymerase chain reaction techniques. Biopsy specimens from five proven verrucous skin lesions were used as positive controls, and peripheral blood samples from five healthy volunteers were used as negative controls.Results: Four test samples were found to have inadequate deoxyribonucleic acid purity and were therefore excluded from the study. Human papilloma virus deoxyribonucleic acid was detected in seven of 95 cases of laryngeal squamous cell carcinoma (7.36 per cent). Human papilloma virus genotyping revealed double human papilloma virus infection in three cases and single human papilloma virus infection in the remaining four cases. The human papilloma virus genotypes detected were 6, 11 and 16 (the latter detected in only one case).Conclusion: In our series, a very low human papilloma virus prevalence was found among laryngeal squamous cell carcinoma cases. The human papilloma virus genotypes detected were mostly 6 and/or 11, and 16 in only one case. To the best of our knowledge, this is the first report of human papilloma virus prevalence in laryngeal squamous cell carcinoma, based on polymerase chain reaction genotyping in a Turkish population.