Quantitative trait locus analysis of ovarian cysts derived from rete ovarii in MRL/MpJ mice
Quantitative trait locus analysis of ovarian cysts derived from rete ovarii in MRL/MpJ mice
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DOI：
10.1007/s00335-010-9254-x
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发表时间：
2010-04-01
期刊：
影响因子：
2.5
通讯作者：
Kon, Yasuhiro
中科院分区：
文献类型：
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作者：
Lee, Shin-hyo;Ichii, Osamu;Kon, Yasuhiro
MRL/MpJ（MRL）是自身免疫性疾病如皮炎、血管炎、关节炎和肾小球肾炎的模型小鼠。除了这些免疫相关的疾病，我们发现，老年MRL小鼠发展卵巢囊肿起源于卵巢网，这是内衬纤毛或无纤毛上皮和中肾小管的残余。据报道，卵巢囊肿有几个来源，与女性不孕症有关，但关于卵巢囊肿起源于卵巢网的遗传病因学信息很少。本研究利用120个微卫星标记和213个MRL小鼠与C57 BL/6 N小鼠回交后代的数量性状位点（QTL），分析了卵巢囊肿发生的遗传背景。测量的数量性状是卵巢网或卵巢囊肿的周长。结果表明，在第3、4、6和11条染色体上检测到了连锁，但在第14条染色体上检测到了显著连锁。因此，我们将Chr 14“MRL网卵巢囊肿（mroc）的27.5-cM区域命名为。“特别是Chrs 4和14的峰区域显示出密切的加性相互作用（p < 0.00001）。从这些结果中，我们得出结论，多个位点上的Chrs 3，4，6，11，和14相互作用，导致在MRL小鼠卵巢囊肿的发展。
MRL/MpJ (MRL) is a model mouse for autoimmune diseases such as dermatitis, vasculitis, arthritis, and glomerulonephritis. In addition to these immune-associated disorders, we found that older MRL mice develop ovarian cysts originating from the rete ovarii, which is lined by ciliated or nonciliated epithelium and considered remnants of mesonephric tubules. Ovarian cysts, which are reported to have several sources, are associated with female infertility, but information regarding the genetic etiology of ovarian cysts originating from the rete ovarii is rare. In this study, to elucidate the genetic background of development of ovarian cysts, we performed quantitative trait locus (QTL) analysis using 120 microsatellite markers, which cover the whole genome of murine chromosomes, and 213 backcross progenies between female MRL and male C57BL/6N mice. The quantitative trait measured was the circumferences of rete ovarii or ovarian cysts. As a result, suggestive linkages were detected on Chrs 3, 4, 6, and 11, but significant linkages were located on Chr 14 by interval mapping. We thereby designated the 27.5-cM region of Chr 14 "MRL Rete Ovarian Cysts (mroc)." The peak regions of Chrs 4 and 14 in particular showed a close additive interaction (p < 0.00001). From these results we concluded that multiple loci on Chrs 3, 4, 6, 11, and 14 interact to result in development of ovarian cysts in MRL mice.