Fluctuation Theorem Applied to F1-ATPase
Fluctuation Theorem Applied to F1-ATPase
复制标题
DOI：
10.1103/physrevlett.104.218103
复制
发表时间：
2010-05-28
影响因子：
8.6
通讯作者：
Noji, Hiroyuki
中科院分区：
文献类型：
--
作者：
Hayashi, Kumiko;Ueno, Hiroshi;Noji, Hiroyuki
近年来，涨落定理（FT）和Jarzynski等式等非平衡统计力学理论已被实验性地应用于微米和纳米系统。然而，到目前为止，这些理论很少应用于运动蛋白等自主系统。特别是，FT 代表了失去平衡的小系统中熵产生的性质，似乎适合应用于它们。因此，我们首次在运动蛋白F-1-三磷酸腺苷（F-1）的单分子实验中使用FT，其中转子γ亚基在三磷酸腺苷水解后在定子α（3）β（3）环中旋转。我们发现 FT 比传统方法能够更好地估计 F-1 的旋转扭矩。
In recent years, theories of nonequilibrium statistical mechanics such as the fluctuation theorem (FT) and the Jarzynski equality have been experimentally applied to micro and nanosized systems. However, so far, these theories are seldom applied to autonomous systems such as motor proteins. In particular, representing the property of entropy production in a small system driven out of equilibrium, FT seems suitable to be applied to them. Hence, for the first time, we employed FT in the single molecule experiments of the motor protein F-1-adenosine triphosphatase (F-1), in which the rotor gamma subunit rotates in the stator alpha(3)beta(3) ring upon adenosine triphosphate hydrolysis. We found that FT provided the better estimation of the rotary torque of F-1 than the conventional method.