Replicative MCM7 protein as a proliferation marker in endometrial carcinoma: a tissue microarray and clinicopathological analysis
Replicative MCM7 protein as a proliferation marker in endometrial carcinoma: a tissue microarray and clinicopathological analysis
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DOI：
10.1111/j.1365-2559.2005.02069.x
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发表时间：
2005-03-01
期刊：
影响因子：
6.4
通讯作者：
Cheung, ANY
中科院分区：
文献类型：
--
作者：
Li, SS;Xue, WC;Cheung, ANY
目的：利用组织微阵列（TMA）技术，利用微小染色体维持（MCM）蛋白（MCM7）评估子宫内膜癌的增殖活性，并探讨其对预后的潜在价值。MCM蛋白是真核DNA复制所必需的，最近已被用来定义在人体tissues.Methods和结果的增殖隔间：免疫组化MCM7和Ki67进行构建从212例子宫内膜癌的TMA。根据核染色的程度定量MCM7和Ki67表达。分析MCM7和Ki67表达与子宫内膜癌临床病理特征的关系。MCM7和Ki67免疫反应性在肿瘤细胞核中明显。子宫内膜癌中MCM7和Ki67标记指数相关（P < 0.001）。MCM7标记指数与组织学分级（P = 0.008）和患者确诊年龄（P < 0.001）相关。高分化癌和年轻患者的MCM7指数较低。MCM7指数高的子宫内膜癌患者生存率低（P = 0.03），多因素分析发现MCM7是一个独立的预后因素（P = 0.04）。Ki67标记指数与组织学分级相关（P = 0.01），但没有显着的预后影响（P = 0.50）。结论：在这个TMA研究子宫内膜癌，MCM7被认为是一个更可靠和有用的标志物比Ki67在评估肿瘤增殖和患者的预后。
Aims: To assess, in tissue microarray (TMA), the proliferative activity of endometrial carcinoma using one of the minichromosome maintenance (MCM) proteins (MCM7), and to explore its potential value for prognosis. MCM proteins are essential for eukaryotic DNA replication and have recently been used to define the proliferative compartments in human tissues.Methods and results: Immunohistochemistry for MCM7 and Ki67 was performed on TMAs constructed from 212 cases of endometrial carcinoma. MCM7 and Ki67 expression was quantified according to the extent of nuclear staining. An analysis was carried out of the association between MCM7 expression and that of Ki67 and the clinicopathological characteristics of endometrial carcinoma. MCM7 and Ki67 immunoreactivity was clearly evident in the nuclei of tumour cells. MCM7 and Ki67 labelling indices in endometrial carcinomas correlated with each other (P < 0.001). A significant correlation existed between the MCM7 labelling index and histological grade (P = 0.008) and patients' age at diagnosis (P < 0.001). Well-differentiated carcinomas and younger patients had a lower MCM7 index. Poor survival was observed in patients with endometrial carcinoma with a high MCM7 index (P = 0.03) and MCM7 was found to be an independent prognostic factor by multivariate analysis (P = 0.04). The Ki67 labelling index correlated with histological grade (P = 0.01) but had no significant prognostic impact (P = 0.50).Conclusions: In this TMA study on endometrial carcinoma, MCM7 was found to be a more reliable and useful marker than Ki67 in assessing tumour proliferation and in the prognosis of patients.