Assessing the diet of a predator using a DNA metabarcoding approach
Assessing the diet of a predator using a DNA metabarcoding approach
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使用 DNA 元条形码方法评估捕食者的饮食
DOI：
10.3389/fevo.2022.902412
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发表时间：
2022-07
影响因子：
3
通讯作者：
Lu Zhang
中科院分区：
文献类型：
--
作者：
Qiaoyun Wang;Zichen Wang;Kaidan Zheng;Peng Zhang;Limin Shen;Wanli Chen;Pengfei Fan;Lu Zhang
顶级捕食者的饮食是确定其生态功能和保护管理所需的重要信息。然而，许多捕食者的难以捉摸的习惯和低人口密度限制了他们的饮食决定。虽然粪便内容物的形态鉴定是传统的方法，DNA元条形码最近被证明是一种更有效和准确的方法来识别猎物类群。利用12 S和16 SrRNA线粒体引物，对淡水生态系统中的顶级捕食者--欧亚水獭（Lutra lutra）的食物进行了DNA元条形码分析。饮食没有不同的数据删除阈值0.1%，1%，3%和5%，包括鱼类（90%），两栖动物和鸟类（2%），偶尔哺乳动物（2%）。12 S和16 S引物揭示了相似的水獭饮食，表明具有较高阈值的单组引物用于检测主要猎物类群具有成本效益。利用12 S引物和5%的阈值，我们发现唐家河国家级自然保护区水獭的食性没有季节性变化。在保护区外发现了不同的猎物群落，这导致了水獭不同的猎物组成。然而，猎物类群的丰富性是没有不同的水獭在保护区内外。水獭喜欢裂腹鱼属，在保护区外，它们主要捕食小型鱼类布氏高原鳅。水獭灵活的摄食策略反映了其高度的适应性。然而，保护区外更大的人为干扰可能会带来重大挑战，水獭改变猎物社区和减少猎物的盈利能力。结合粪便DNA元条形码和当地鱼类调查将提供机会，更详细的研究不同程度的人类干扰对猎物群落和水獭的影响。
The diet of top predators is vital information needed to determine their ecological function and for their conservation management. However, the elusive habit and low population density of many predators constrains determination of their diets. While the morphological identification of scat contents is the traditional method, DNA metabarcoding has lately proven a more efficient and accurate method of identifying prey taxa. We applied DNA metabarcoding to analyzing the diet of the Eurasian otter ( Lutra lutra ), a top predator in freshwater ecosystems, using 12S and 16S rRNA mitochondrial primers target vertebrate prey. Diet did not vary among different data removal thresholds of 0.1, 1, 3, and 5%, comprising fishes (90%), amphibians and birds (2%), and occasionally mammals (2%). Both 12S and 16S primers revealed similar otter diets, indicating that a single set of primers with a higher threshold is cost-effective for detecting the main prey taxa. Using 12S primers and a 5% threshold, we found no seasonal variation of otter diet in the Tangjiahe National Nature Reserve. A different prey community was found outside the reserve, which resulted in different prey composition for otters. However, prey taxon richness was not different between otters in- and outside the reserve. Otters preferred Schizothorax spp., the largest-sized fish species in the reserve, whereas they mainly preyed on Triplophysa bleekeri , a small-sized fish species, outside the reserve. Otters’ flexible feeding strategy reflect their high adaptability. However, greater human disturbance outside the reserve may present significant challenges to otters by altering prey communities and reducing prey profitability. Combining fecal DNA metabarcoding and local fish survey will provide opportunities for more detailed studies on the impact of different levels of human disturbances on prey communities and otters.
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影响因子：
3.5
作者：
Miya M;Sato Y;Fukunaga T;Sado T;Poulsen JY;Sato K;Minamoto T;Yamamoto S;Yamanaka H;Araki H;Kondoh M;Iwasaki W
通讯作者：
Iwasaki W
影响因子：
7.7
作者：
Thalinger B;Oehm J;Mayr H;Obwexer A;Zeisler C;Traugott M
通讯作者：
Traugott M
影响因子：
--
作者：
L. Remonti;C. Prigioni;A. Balestrieri;S. Sgrosso;G. Priore
通讯作者：
L. Remonti;C. Prigioni;A. Balestrieri;S. Sgrosso;G. Priore
影响因子：
4.9
作者：
Deagle BE;Thomas AC;McInnes JC;Clarke LJ;Vesterinen EJ;Clare EL;Kartzinel TR;Eveson JP
通讯作者：
Eveson JP
DOI：
10.1093/bioinformatics/bts565
发表时间：
2012-12-01
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Fu L;Niu B;Zhu Z;Wu S;Li W
通讯作者：
Li W