A Multi-Functional Silicon Nanoparticle Designed for Enhanced Osteoblast Calcification and Related Combination Therapy
A Multi-Functional Silicon Nanoparticle Designed for Enhanced Osteoblast Calcification and Related Combination Therapy
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一种多功能硅纳米颗粒，旨在增强成骨细胞钙化和相关联合治疗
DOI：
10.1002/mabi.201900255
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发表时间：
2019
影响因子：
4.6
通讯作者：
Huang Yubin
中科院分区：
文献类型：
--
作者：
Shao Nannan;Guan Yuyao;Liu Sha;Li Xiaoyuan;Zhou Dongfang;Huang Yubin
目前应用于骨缺损修复的植入材料多侧重于诱导骨再生，而忽视了对细菌感染、炎症等并发症的治疗，这些并发症可能导致骨愈合延迟甚至截肢。在本研究中，合成了微孔二氧化硅纳米颗粒-聚（N-异丙基丙烯酰胺-B-（2-（二甲氨基）乙基甲基丙烯酸酯），用于负载DXMS和ECM衍生肽（序列：琥珀酸-GTPGPQGIAGQRGVV），以增强成骨细胞钙化并缓解相关症状。带正电荷的PDMA嵌段赋予纳米颗粒抗微生物性质。此外，DXMS的组合使其具有抗炎和促进钙化形成的能力。此外，肽的掺入导致前成骨细胞中矿化和碱性磷酸酶表达的显著改善。在小鼠肌肉内植入四周后，结果表明复合纳米颗粒可以促进异位骨形成。这些综合性能使复合硅纳米颗粒成为一种有前途的成骨药物，适合于骨修复和相关联合治疗的进一步研究。
Implant materials applied in bone defect commonly focus on the inducement of bone regeneration and neglect to cure complications including bacterial infection and inflammation, which may result in delayed unions or even amputation. In this study, a microporous silica nanoparticle‐poly(N‐isopropylacrylamide‐b‐(2‐(dimethylamino)ethyl methacrylate) is synthesized for loading DXMS and the ECM‐derived peptide (Sequence: Succinic acid‐GTPGPQGIAGQRGVV) in order to enhance the osteoblast calcification and relieve related symptoms. Positively charged PDMA blocks endow the nanoparticle with the antimicrobial property. Moreover, the combination of DXMS makes it have the ability of anti‐inflammation and promoting calcification formation. Furthermore, incorporation of the peptide leads to a significant improvement of mineralization and alkaline phosphatase expression in the preosteoblast. After intramuscular implantation in mice for four weeks, the results indicate the composite nanoparticle can promote ectopic bone formation. These combined properties make the composite silicon nanoparticle a promising osteogenic drug appropriate for further study in bone repair and related combination therapy.