Human retinal pigment epithelial cells express scavenger receptors BI and BII
Human retinal pigment epithelial cells express scavenger receptors BI and BII
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DOI：
10.1006/bbrc.2002.6756
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发表时间：
2002-04-12
影响因子：
3.1
通讯作者：
Schwartz, DM
中科院分区：
文献类型：
--
作者：
Duncan, KG;Bailey, KR;Schwartz, DM
视网膜色素上皮（RPE）细胞是视网膜的基础，每天摄取和代谢数千个富含脂质的光感受器外节（POS）。清道夫受体（SR）CD 36和整联蛋白α v β 5已显示参与POS结合和RPE细胞的内化。本研究的目的是确定RPE细胞是否表达除CD 36以外的SR。人RPE细胞的原代培养物表达SR-BI和SR-BII的mRNA和蛋白质。用逆转录-聚合酶链反应检测SR-BI和SR-BII mRNA。通过[S-35]蛋氨酸标记的粗细胞提取物的免疫沉淀检测SR-BI蛋白。通过免疫沉淀的粗细胞提取物的Western印迹检测SR-BII。培养的RPE细胞免疫荧光染色也检测到SR-BI和SR-BII蛋白。结果表明，这些SR可能在POS脂质结合和RPE细胞在眼睛中的摄取中发挥作用。(C)2002 Elsevier Science（美国）。
The retinal pigment epithelial (RPE) cells that underlie the retina ingest and metabolize thousands of lipid-rich photoreceptor outer segments (POS) every day. The scavenger receptor (SR) CD36 and integrin alphavbeta5 have been shown to participate in POS binding and internalization by RPE cells. The objective of the current study was to determine whether RPE cells express SRs other than CD36. Primary cultures of human RPE cells express both mRNA and protein for SR-BI and SR- BII. SR-BI and SR-BII mRNAs were detected by reverse transcription-polymerase chain reaction. SR-BI protein was detected by immune precipitation of [S-35]methionine-labeled crude cellular extracts. SR-BII was detected by Western blotting of immune precipitated crude cellular extracts. SR-BI and SR-BII proteins were also detected by immunofluorescence staining of RPE cells in culture. The results suggest that these SRs may play a role in POS lipid binding and uptake by RPE cells in the eye. (C) 2002 Elsevier Science (USA).