Antidepressant‐like effects of Sanyuansan in the mouse forced swim test, tail suspension test, and chronic mild stress model
Antidepressant‐like effects of Sanyuansan in the mouse forced swim test, tail suspension test, and chronic mild stress model
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DOI：
10.1016/j.kjms.2015.10.009
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发表时间：
2015-12
期刊：
影响因子：
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通讯作者：
Shuo Yan;Zili You;Qiuying Zhao;Cheng Peng;Gang He;X. Gou;Bin Lin
中科院分区：
文献类型：
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作者：
Shuo Yan;Zili You;Qiuying Zhao;Cheng Peng;Gang He;X. Gou;Bin Lin
天然产物已被广泛报道为治疗抑郁症的有效治疗替代品。三元散是由人参总皂苷、鱼油、缬草等组成的复方制剂。本研究通过强迫游泳实验（FST）、悬尾实验（TST）、自发活动实验和慢性轻度应激（CMS）小鼠抑郁模型，验证三元散是否具有抗抑郁样作用。C57 BL/6小鼠分别经口给予丙咪嗪30 mg/kg、三元散30 mg/kg和生理盐水。给药后连续3次（给药前24 h、1 h和0.5 h）进行急性行为学试验，包括TST、FST和自发活动试验。此外，对CMS模型小鼠的蔗糖偏好性和血清皮质酮水平进行了检测。与生理盐水处理组相比，三元散900 mg/kg显著减少TST小鼠的不动时间。三元散225 mg/kg、450 mg/kg、900 mg/kg能显著缩短小鼠不动时间。三元散可逆转CMS所致的快感缺失和下丘脑-垂体-肾上腺轴的过度激活。此外，我们的研究结果表明，丙咪嗪和三元散在任何剂量都没有增加自发运动活动。提示三元散在急性和慢性动物模型中均具有明显的抗抑郁样作用，但其作用机制可能与增加小鼠的自主活动有关，且无镇静样作用。
Natural products have been widely reported as effective therapeutic alternatives for treatment of depression. Sanyuansan is a compound recipe composed of ginseng total saponins, fish oil, and valeriana. The aims of this study were to validate whether Sanyuansan has antidepressant-like effects through acute behavioral tests including the forced swimming test (FST), tail suspension test (TST), locomotor activity test, and chronic mild stress (CMS) mice model of depression. C57BL/6 mice were given oral administration of 30 mg/kg imipramine, Sanyuansan, and saline, respectively. The acute behavioral tests including the TST, FST, and locomotor activity test were done after the administration of drugs for consecutively three times (24 hours, 1 hour, and 0.5 hour prior to the tests). Furthermore, the sucrose preference and the serum corticosterone level of mice in the CMS model were examined. Sanyuansan only at 900 mg/kg markedly reduced immobility time in the TST compared with the saline-treated group of mice. Sanyuansan at doses of 225 mg/kg, 450 mg/kg, and 900 mg/kg significantly reduced immobility time of mice in the FST. Sanyuansan reversed the CMS-induced anhedonia and hyperactivation of the hypothalamus–pituitary–adrenal axis. In addition, our results showed that neither imipramine nor Sanyuansan at any dosage increased spontaneous motor activity. These results suggested that Sanyuansan induced significant antidepressant-like effects in mice in both acute and chronic animal models, which seemed unlikely to be attributed to an increase in locomotor activities of mice, and had no sedative-like effects.