Tobacco rattle virus-induced gene silencing in Hevea brasiliensis
Tobacco rattle virus-induced gene silencing in Hevea brasiliensis
复制标题
烟草脆裂病毒诱导的巴西橡胶基因沉默
DOI：
10.1093/bbb/zbaa085
复制
发表时间：
2021
影响因子：
1.6
通讯作者：
Peng Shi-Qing
中科院分区：
文献类型：
--
作者：
Li Hui-Liang;Guo Dong;Wang Ying;Zhu Jia-Hong;Qu Long;Peng Shi-Qing
病毒诱导的基因沉默（VIGS）是一种在植物中广泛应用的基因沉默工具。据了解，VIGS在橡胶树上的应用尚未见报道。本研究利用VIGS技术对橡胶树进行了有效的基因沉默。在橡胶树基因组中鉴定出编码橡胶树叶烯脱氢酶（HbPDS）的基因。预测了来自HbPD的小干扰RNA和沉默基因片段，并选择了399 bp的长度进行测试。结果表明，烟草脆裂病毒（TRV）-VIGS能有效地诱导橡胶树HbPDS基因沉默。本研究首次报道了橡胶树VIGS。该方法可用于橡胶树功能基因的反向遗传鉴定，并可进一步用于橡胶树基因沉默植株的培育，促进橡胶树功能基因的研究。
Virus-induced gene silencing (VIGS) is a powerful gene-silencing tool that has been intensively applied in plants. To data, the application of VIGS in rubber tree has not yet been reported. In this study, we described the efficient gene silencing in rubber tree by VIGS. The gene encodingHevea brasiliensisphytoene desaturase (HbPDS) was identified in rubber tree genome. Small interfering RNAs fromHbPDSand the silencing gene fragment were predicted and a length of 399 bp was selected to be tested. We showed that the tobacco rattle virus (TRV)-VIGS could induce effectiveHbPDSsilencing in rubber tree. This study was the first to report VIGS in rubber tree. The present TRV-VIGS method could be used to perform reverse genetic approaches to identify unknown gene functions and might be further applied to produce gene silenced rubber tree plants, to advance functional gene of rubber tree.