Development of a genomic microsatellite library in perennial ryegrass (Lolium perenne) and its use in trait mapping
Development of a genomic microsatellite library in perennial ryegrass (Lolium perenne) and its use in trait mapping
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DOI：
10.1093/aob/mcn016
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发表时间：
2008-04-01
期刊：
影响因子：
4.2
通讯作者：
King, I. P.
中科院分区：
文献类型：
--
作者：
King, J.;Thorogood, D.;King, I. P.
背景与目的多年生黑麦草（Lolium perenne）是世界上重要的饲草和观赏牧草之一。在英国，它占所有农业用地的70%，每年的农场产值估计为60亿英镑。然而，在遗传资源方面，二年生黑麦草相对于其他禾本科主要作物来说是落后的。因此，该项目的目的是建立一个富含微卫星的羊草基因组文库，以增加遗传标记的数量，用于育种计划中的标记辅助选择和基因分离。方法设计229个非冗余微卫星标记引物，筛选丰美型和草料型两种小麦基因型。在229颗微卫星中，有95颗在舒适型和饲料型之间存在多态性。从这95个微卫星引物中筛选了两个定位群体，这些群体来自于两种牧草和舒适草基因型的杂交和回交。通过将抽穗日期相关基因定位到连锁群4和连锁群7，证明了所得到的遗传图谱在分析目标性状遗传控制方面的实用性。
Background and Aims Perennial ryegrass (Lolium perenne) is one of the key forage and amenity grasses throughout the world. In the UK it accounts for 70 % of all agricultural land use with an estimated farm gate value of 6 pound billion per annum. However, in terms of the genetic resources available, L. perenne has lagged behind other major crops in Poaceae. The aim of this project was therefore the construction of a microsatellite-enriched genomic library for L. perenne to increase the number of genetic markers available for both marker-assisted selection in breeding programmes and gene isolation.Methods Primers for 229 non-redundant microsatellite markers were designed and used to screen two L. perenne genotypes, one amenity and one forage. Of the 229 microsatellites, 95 were found to show polymorphism between amenity and forage genotypes. A selection of microsatellite primers was selected from these 95 and used to screen two mapping populations derived from intercrossing and backcrossing the two forage and amenity grass genotypes.Key Results and Conclusions The utility of the resulting genetic maps for analysis of the genetic control of target traits was demonstrated by the mapping of genes associated with heading date to linkage groups 4 and 7.