Reclassification of Lactobacillus casei subsp casei ATCC 393 and Lactobacillus rhamnosus ATCC 15820 as Lactobacillus zeae nom rev, designation of ATCC 334 as the neotype of L-casei subsp casei, and rejection of the Lactobacillus paracasei
Reclassification of Lactobacillus casei subsp casei ATCC 393 and Lactobacillus rhamnosus ATCC 15820 as Lactobacillus zeae nom rev, designation of ATCC 334 as the neotype of L-casei subsp casei, and rejection of the Lactobacillus paracasei
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DOI：
10.1099/00207713-46-1-337
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发表时间：
1996-01-01
期刊：
影响因子：
--
通讯作者：
Lauer, E
中科院分区：
文献类型：
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作者：
Dicks, LMT;DuPlessis, EM;Lauer, E
干酪乳杆菌亚种的类型菌株。casei （ATCC 393）与其他菌株的DNA同源性较低。casei（8 ~ 46%）和palacasei乳杆菌（30 ~ 50%），但与“玉米乳杆菌”的原始型菌株鼠李糖乳杆菌ATCC 15820的DNA相似性为80%。菌株ATCC 393(T) （T =型菌株）和ATCC 15820(T)是一个蛋白质谱簇的成员，与其他乳杆菌属分离，菌株ATCC 393(T)的随机扩增多态性DNA PCR谱也与其他种不同。干酪乳杆菌ATCC 334(T)与干酪乳杆菌亚种有亲缘关系。干酪乳杆菌菌株（71 ~ 97%）和副干酪乳杆菌菌株（71 ~ 91%）与这些生物是同一蛋白谱簇的成员，并且与副干酪乳杆菌菌株具有相同的DNA扩增子。在此基础上，我们提出干酪乳杆菌亚种。casei ATCC 393(T)和L. rhamnosus ATCC 15820应重新归类为玉米乳杆菌（型菌株，ATCC 15820）的成员，该菌株ATCC 334应被指定为casei亚种的新型菌株。因此，应拒绝将其命名为L. parcasei。
The type strain of Lactobacillus casei subsp. casei (ATCC 393) exhibits low levels of DNA homology with other strains of L. casei subsp. casei (8 to 46%) and strains of Lactobacillus palacasei (30 to 50%), but exhibits a level of DNA similarity of 80% with Lactobacillus rhamnosus ATCC 15820, the original type strain of ''Lactobacterium zeae'' Kuznetsov 1959. Strains ATCC 393(T) (T = type strain) and ATCC 15820(T) are members of one protein profile cluster that is separate from the other Lactobacillus spp. The randomly amplified polymorphic DNA PCR profile of strain ATCC 393(T) is also different from the profiles obtained for the other species. L. casei ATCC 334(T) is genetically closely related to L. casei subsp. casei strains (71 to 97%) and L. paracasei strains (71 to 91%), is a member of the same protein profile cluster as these organisms, and shares several DNA amplicons with L. paracasei strains. On the basis of these results, we propose that L. casei subsp. casei ATCC 393(T) and L. rhamnosus ATCC 15820 should be reclassified as members of lactobacillus zeae nom. rev. (type strain, ATCC 15820), that strain ATCC 334 should be designated the neotype strain oft, casei subsp. casei, and that the name L. paracasei should be rejected.