Direct measurement of the mechanical properties of a chromatin analog and the epigenetic effects of para-sulphonato-calix[4]arene
Direct measurement of the mechanical properties of a chromatin analog and the epigenetic effects of para-sulphonato-calix[4]arene
复制标题
直接测量染色质类似物的机械特性和对磺基杯[4]芳烃的表观遗传效应
DOI：
10.1038/s41598-019-42267-x
复制
发表时间：
2019
影响因子：
4.6
通讯作者：
Hiroyuki Fujita & Anthony W. Coleman
中科院分区：
文献类型：
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作者：
Yannick Tauran;Momoko Kumemura;Mehmet C. Tarhan;Gregoire Perret;Florent Perret;Laurent Jalabert;Dominique Collard;Hiroyuki Fujita & Anthony W. Coleman
利用硅纳米镊子（SNTs）研究了λ噬菌体DNA与小牛胸腺核小体相互作用形成人工染色质类似物过程中对DNA力学性质的影响。在100 nM的浓度下，核小体溶液诱导了对DNA的强烈硬化效应（1.1 N m−1）。这可以与组蛋白H1、H2 A、H3的作用进行比较，其中没有观察到DNA的机械性质的变化，并且与H3/H4蛋白的复合物的作用进行比较，其中观察到刚度的较小增加（0.2 N m−1）.对磺酸基杯[4]芳烃，SC 4，已知通过与组蛋白的赖氨酸基团特异性相互作用而具有表观遗传活性，研究了其对人工染色质的影响。使用微流控SNT装置，针对人工染色质滴定SC 4，在SC 4中浓度为1 mM时，观察到刚度显著增加，15 N m-1。同时，光学显微镜显示SNT装置尖端之间的DNA结构发生了物理变化。电子和原子力显微镜证实了这种结构重排。阴性对照实验证实，这些机械和物理效应既不是由SC 4的酸度引起的，也不是通过SC 4对DNA的非特异性相互作用引起的。
By means of Silicon Nano Tweezers (SNTs) the effects on the mechanical properties of λ-phage DNA during interaction with calf thymus nucleosome to form an artificial chromatin analog were measured. At a concentration of 100 nM, a nucleosome solution induced a strong stiffening effect on DNA (1.1 N m−1). This can be compared to the effects of the histone proteins, H1, H2A, H3 where no changes in the mechanical properties of DNA were observed and the complex of the H3/H4 proteins where a smaller increase in the stiffness is observed (0.2 N m−1).Para-sulphonato-calix[4]arene, SC4, known for epigenetic activity by interacting specifically with the lysine groups of histone proteins, was studied for its effect on an artificial chromatin. Using a microfluidic SNT device, SC4 was titrated against the artificial chromatin, at a concentration of 1 mM in SC4 a considerable increase in stiffness, 15 N m−1, was observed. Simultaneously optical microscopy showed a physical change in the DNA structure between the tips of the SNT device. Electronic and Atomic Force microscopy confirmed this structural re-arrangement. Negative control experiments confirmed that these mechanical and physical effects were induced neither by the acidity of SC4 nor through nonspecific interactions of SC4 on DNA.