Expression of Genes Related to Germ Cell Lineage and Pluripotency in Single Cells and Colonies of Human Adult Germ Stem Cells
Expression of Genes Related to Germ Cell Lineage and Pluripotency in Single Cells and Colonies of Human Adult Germ Stem Cells
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DOI：
10.1155/2016/8582526
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发表时间：
2016-01-01
影响因子：
4.3
通讯作者：
Skutella, Thomas
中科院分区：
文献类型：
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作者：
Conrad, Sabine;Azizi, Hossein;Skutella, Thomas
本研究的目的是阐明单个人类成体生殖干细胞 (haGSC) 和 haGSC 集落的分子状态，这些集落由 CD49f MACS 和基质（胶原蛋白/层粘连蛋白+结合）选择的富集精原细胞部分自发发育而成。 Fluidigm BioMark 系统对长期培养的 haGSC 簇进行的单细胞转录分析与人胚胎干细胞 (hESC) 和人成纤维细胞 (hFib) 的比较表明，haGSC 表现出特征性的生殖和多能性相关基因表达谱，与 hESC 有一些相似之处，但与体细胞 hFib 存在显着区别。与微阵列分析的全基因组比较证实，不同的 haGSC 集落表现出基因表达异质性，或多或少具有多能性。这项研究的结果证实，haGSC 是体外具有特定分子基因表达谱的成体干细胞，与真正的多能干细胞相关但不完全相同。在ES细胞条件下，可以选择haGSC集落并在分子水平上维持部分多能状态，这可能与其细胞可塑性和分化为所有胚层细胞的潜力有关。
The aim of this study was to elucidate the molecular status of single human adult germ stem cells (haGSCs) and haGSC colonies, which spontaneously developed from the CD49f MACS and matrix- (collagen-/laminin+ binding-) selected fraction of enriched spermatogonia. Single-cell transcriptional profiling by Fluidigm BioMark system of a long-term cultured haGSCs cluster in comparison to human embryonic stem cells (hESCs) and human fibroblasts (hFibs) revealed that haGSCs showed a characteristic germ- and pluripotency-associated gene expression profile with some similarities to hESCs and with a significant distinction from somatic hFibs. Genome-wide comparisons with microarray analysis confirmed that different haGSC colonies exhibited gene expression heterogeneity with more or less pluripotency. The results of this study confirm that haGSCs are adult stem cells with a specific molecular gene expression profile in vitro, related but not identical to true pluripotent stem cells. Under ES-cell conditions haGSC colonies could be selected and maintained in a partial pluripotent state at the molecular level, which may be related to their cell plasticity and potential to differentiate into cells of all germ layers.