Proteomic Analysis of Ribosomes: Translational Control of mRNA Populations by Glycogen Synthase GYS1
Proteomic Analysis of Ribosomes: Translational Control of mRNA Populations by Glycogen Synthase GYS1
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DOI：
10.1016/j.jmb.2011.04.064
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发表时间：
2011-07-01
影响因子：
5.6
通讯作者：
Sarnow, Peter
中科院分区：
文献类型：
--
作者：
Fuchs, Gabriele;Diges, Camille;Sarnow, Peter
核糖体是由核糖体RNA分子、核糖体蛋白和许多相关的“非核糖体”蛋白组成的大分子复合物的异质池。为了鉴定可能调节核糖体活性的非核糖体蛋白，我们使用蛋白质组学多维蛋白鉴定技术检测了翻译活性和非翻译活性核糖体的组成。值得注意的是，糖原合成酶的磷酸化异构体糖原合成酶1 （GYS1）优先与延长核糖体相关。GYS1的缺失影响了一部分细胞mrna的翻译，其中一些mrna编码调节蛋白质生物合成的蛋白质。这些发现表明，GYS1的丰度，凭借其核糖体关联，在细胞的能量状态和翻译机制之间提供了一个反馈回路。(C) 2011 Elsevier Ltd.版权所有。
Ribosomes exist as a heterogenous pool of macromolecular complexes composed of ribosomal RNA molecules, ribosomal proteins, and numerous associated "nonribosomal" proteins. To identify nonribosomal proteins that may modulate ribosome activity, we examined the composition of translationally active and inactive ribosomes using a proteomic multidimensional protein identification technology. Notably, the phosphorylated isoform of glycogen synthase, glycogen synthase 1 (GYS1), was preferentially associated with elongating ribosomes. Depletion of GYS1 affected the translation of a subset of cellular mRNAs, some of which encode proteins that modulate protein biosynthesis. These findings argue that GYS1 abundance, by virtue of its ribosomal association, provides a feedback loop between the energy state of the cells and the translation machinery. (C) 2011 Elsevier Ltd. All rights reserved.