EFFECT OF TRAINING AND EXHAUSTION ON HEXOKINASE ACTIVITY OF SKELETAL MUSCLE
EFFECT OF TRAINING AND EXHAUSTION ON HEXOKINASE ACTIVITY OF SKELETAL MUSCLE
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DOI：
10.1152/jappl.1969.27.5.691
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发表时间：
1969-01-01
影响因子：
3.3
通讯作者：
PETER, JB
中科院分区：
文献类型：
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作者：
BARNARD, RJ;PETER, JB
巴纳德河詹姆斯，和JB彼得。训练和力竭对骨骼肌己糖激酶活性的影响。应用生理学杂志27（5）：691-695。1969.-本文研究了不同动态运动方案和运动至力竭对豚鼠骨骼肌匀浆中己糖激酶活性的影响。在以1.3 km/hr的速度进行30 min/天的跑步机跑步9天后，己糖激酶活性大约加倍。此后，运动增加到每天两次，每次30分钟，己糖激酶显著降低。停止训练后，己糖激酶活性呈指数下降，约12天后达到对照-久坐水平。当训练过的动物跑得筋疲力尽时，己糖激酶活性显著下降。在总己糖激酶和可溶性己糖激酶中均观察到这些反应。豚鼠每周运动6天，每天运动5分钟，每天运动15分钟，每隔一天运动30分钟。4周后，所有三种训练方案的己糖激酶活性大约加倍。然而，对于5和15分钟/天组，分别从40 rt 4到77= t 13 min运行到耗尽。它的结论是，己糖激酶活性是非常敏感的急性和慢性运动，但引起的变化不一定与训练状态。锻炼;醛缩酶本实验室的先前研究（3，4）表明，成年雄性豚鼠骨骼肌的可溶性己糖激酶活性随着动态运动而增加。在“红色”和“白色”肌肉中均观察到活性增加。经过3周的训练后，与久坐不动的对照动物相比，活动增加了1.8倍。与通常的30分钟运动相比，训练过的动物的力竭性跑步（80分钟）使己糖激酶活性显著降低。可溶性己糖激酶（105，000 X g上清液）约占我们实验室使用的酶联免疫吸附法1的骨骼肌己糖激酶总活性的30%。因此，本研究旨在进一步探讨运动和力竭对骨骼肌己糖激酶的影响。这些实验旨在回答以下问题。不同强度的动态运动对己糖激酶活性有什么影响。3 2）训练动物的疲劳对总己糖激酶活性有什么影响？第三章
BARNARD, R. JAMES, AND JB PETER. Effect of training and exhaustion on hexokinase activity of skeletal muscle. J. Appl. Physiol. 27 (5): 691-695. 1969.-The effects of various dynamic exercise programs and of exercise to exhaustion on hexokinase activity in homogenates of guinea pig skeletal muscle were investigated. Hexokinase activity approximately doubled after 9 days of treadmill running at 1.3 km/hr for 30 min/day. Thereafter, exercise was increased to two 30-min bouts/day and hexokinase decreased significantly. After cessation of training the hexokinase activity decreased exponentially, reaching the control-sedentary level after approximately 12 days. When trained animals were run to exhaustion, hexokinase activity decreased significantly. These responses were observed in both the total and soluble hexokinase. Guinea pigs were also exercised 6 days/week for 5 min/day, 15 min/day, or 30 min every other day. After 4 weeks the hexokinase activity approximately doubled with all three training programs. However, the running times to exhaustion ranged from 40 rt 4 to 77= t 13 min for the 5 and 15 min/day groups, respectively. It is concluded that hexokinase activity is very responsive to acute and chronic exercise but the changes induced do not necessarily correlate with the state of training. exercise; aldolasePREVIOUS INVESTIGATIONS(3, 4) from this laboratory have shown that soluble hexokinase activity of skeletal muscle increases with dynamic exercise in adult male guinea pigs. The increase in activity was observed in both “red” and “white” muscle. After 3 weeks of training the activity had increased 1.8-fold compared to the sedentary control animals. An exhaustive run (80 min) in the trained animals produced a significant decrease in hexokinase activity when compared to the usual 30-min bout of exercise. Soluble hexokinase (105,000 X g supernatant) represents approximately 30% of the total activity of skeletal muscle hexokinase with the homogenizing techniques1 used in our laboratory. Therefore, the present study was conducted to investigate further the effects of exercise and exhaustion on skeletal muscle hexokinase. The experiments were designed to answer the following questions. I> What effect do various amounts of dynamic exercise have on hexokinase activity. 3 2) What effect does exhaustion in trained animals have on total hexokinase activity? 3)