Central administration of melanin-concentrating hormone (MCH) suppresses food intake, but not locomotor activity, in the goldfish, Carassius auratus
Central administration of melanin-concentrating hormone (MCH) suppresses food intake, but not locomotor activity, in the goldfish, Carassius auratus
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DOI：
10.1016/j.neulet.2006.02.005
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发表时间：
2006-05
影响因子：
2.5
通讯作者：
K. Matsuda;S. Shimakura;K. Maruyama;T. Miura;M. Uchiyama;H. Kawauchi;S. Shioda;A. Takahashi
中科院分区：
文献类型：
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作者：
K. Matsuda;S. Shimakura;K. Maruyama;T. Miura;M. Uchiyama;H. Kawauchi;S. Shioda;A. Takahashi
黑色素浓缩激素(MCH)是一种下丘脑-脑垂体肽，最初在鲑鱼脑下垂体中被鉴定为一种影响体色的激素。近年来，MCH参与了哺乳动物摄食行为和能量平衡的调节。然而，尽管关于哺乳动物中的MCH的知识越来越多，但关于MCH对鱼类的食欲和行为的影响的信息很少。本研究旨在探讨MCH对金鱼摄食行为和自发活动的影响。我们通过脑室(Icv)注射合成的MCH，并使用自动监测系统检测其对食物摄入量和运动活动的影响。我们使用的两种类型的人工合成MCH，都来自鱼类和人类，都能有效地刺激金鱼鳞片中黑色素颗粒的聚集。脑室注射两种MCHS可显著降低累积摄食量，且呈剂量依赖关系。在观察期间，侧脑室注射与用于检查食物摄入量的相同剂量的鱼类MCH，没有引起明显的运动活动变化。这些结果表明，MCH影响金鱼的摄食行为，但不影响自发的运动活动，并且可能与其在哺乳动物中的厌氧作用不同，在金鱼的大脑中发挥厌氧作用。
Melanin-concentrating hormone (MCH) is a hypothalamo-pituitary peptide, which was first identified in the salmon pituitary as a hormone affecting body color. Recently, MCH has been implicated in the regulation of feeding behavior and energy homeostasis in mammals. Despite a growing body of knowledge concerning MCH in mammals, however, there is little information about the effect of MCH on appetite and behavior in fish. The aim of the present study was to investigate the action of MCH on feeding behavior and spontaneous locomotor activity in the goldfish. We administered synthetic MCH by intracerebroventricular (ICV) injection and examined its effect on food intake and locomotor activity using an automatic monitoring system. Both types of synthetic MCH we employed, which are of fish and human origin, were effective in stimulating aggregation of melanin granules in the melanophores of goldfish scales. Cumulative food intake was significantly decreased by ICV injection of both MCHs in a dose-dependent manner. ICV injection of fish MCH at the same doses as those used for examination of food intake induced no marked changes in locomotor activity during the observation period. These results suggest that MCH influences feeding behavior, but not spontaneous locomotor activity, in the goldfish, and may exert an anorexigenic action in the goldfish brain, unlike its orexigenic action in mammals.