Effects of metal binding to mismatched base pairs on DNA-mediated charge transfer
Effects of metal binding to mismatched base pairs on DNA-mediated charge transfer
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DOI：
10.1016/j.jinorgbio.2007.04.005
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发表时间：
2007-07-01
影响因子：
3.9
通讯作者：
Nishimoto, Sei-ichi
中科院分区：
文献类型：
--
作者：
Ito, Takeo;Nikaido, Go;Nishimoto, Sei-ichi
用聚丙烯酰胺凝胶电泳研究了带有胞嘧啶-胞嘧啶（CC）或胸腺嘧啶-胸腺嘧啶（TT）错配碱基对的DNA双链体中的光诱导空穴转移反应。银（I）离子与CC错配碱基对的位点特异性结合以及与DNA中多个核碱基位点的非特异性结合抑制了空穴通过序列的迁移。在汞（II）的情况下，结合到双链DNA含有单一TT错配在N3的嘧啶环，观察到的空穴传输效率的影响不大，这是根据最近的理论预测。另一方面，除了Hg（II）的双链体含有串联TT碱基对显着降低空穴传输效率，虽然计算表明，这种结合可以形成高度的电子耦合之间的空穴载体基地。(c)2007年爱思唯尔公司All rights reserved.
Photoinduced hole transfer reaction in DNA duplex bearing cytosine-cytosine (CC) or thymine-thymine (TT) mismatched base pairs as metal-ion binding sites was studied using polyacrylamide gel electrophoresis. Site-specific binding of silver (I) ion to a CC mismatched base pair as well as non-specific binding to multiple sites of nucleobases in the DNA suppressed hole migration through the sequence. In the case of mercury (II) binding to duplex DNA containing single TT mismatch at N3 of the pyrimidine rings, little effect on the efficiency of hole transfer was observed, which is in accordance with a recent theoretical prediction. On the other hand, addition of Hg(II) to duplex containing tandem TT base pairs remarkably reduced hole transfer efficiency, although the calculation has suggested such binding could form high degree of electronic coupling between the hole carrier bases. (c) 2007 Elsevier Inc. All rights reserved.