Micro-stamped surfaces for the patterned growth of neural stem cells
Micro-stamped surfaces for the patterned growth of neural stem cells
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DOI：
10.1016/j.biomaterials.2008.08.017
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发表时间：
2008-12-01
期刊：
影响因子：
14
通讯作者：
Ross, Francois
中科院分区：
文献类型：
--
作者：
Ruiz, Ana;Buzanska, Leonora;Ross, Francois
我们提出了一种基于细胞排斥表面（聚环氧乙烷样、PEO 样等离子体沉积膜）上的预图案化多肽的神经干细胞图案化方法。该方法可确保细胞附着和稳定性数周，并允许细胞迁移和分化。使用不同的阵列配置和印刷条件，通过微接触印刷，在细胞排斥性 PEO 样基质上创建了类似于 1 nm 厚的细胞粘合剂聚 L-赖氨酸 (PLL) 的各种图案。 PEO 类薄膜的细胞排斥特性决定了细胞在印刷图案上的限制。打印方法的优化表明，使用在 pH 8.4 的碳酸盐缓冲液 (100 mm) 中稀释的 PLL 可以获得大面积上最均匀的图案。在低血清和分化培养基中以 PLL 模式培养超过 20 天的神经干细胞表现出对多肽结构域的良好限制。附着的细胞数量随着微冲压 PLL 面积线性增加。这些细胞能够将随机的轴突样投射延伸到图案的外部，并在分化培养基中培养时呈现出大量的分支。迁移和轴突样生长已通过互连的正方形配置成功引导。该表面适合控制干细胞的图案，并提供一个平台来评估不同的细胞排列和培养条件如何影响细胞相互作用和细胞发育过程。 (c) 2008 Elsevier Ltd. 保留所有权利。
We present a method for patterning neural stem cells based on pre-patterning polypeptides on a cell-repellent surface (poly(ethylene) oxide-like, PEO-like, plasma-deposited films). The method ensures cell attachment and stability for several weeks, as well as it allows cell migration and differentiation. Various patterns of similar to 1 nm thick cell adhesive poly-L-lysine (PLL) have been created on a cell-repellent PEO-like matrix by microcontact printing using different array configurations and printing conditions. The cell-repellent property of PEO-like film determined the confinement of the cells on the printed patterns. Optimization of the printing method showed that the most homogeneous patterns over large areas were obtained using PLL diluted in carbonate buffer (100 mm) at pH 8.4. Neural stem cells cultured on the PLL patterns in low serum and in differentiating medium over 20 days exhibited a good confinement to the polypeptide domains. The number of cells attached increased linearly with the micro-stamped PLL area. The cells were able to extend random axon-like projections to the outside of the patterns and presented high amount of ramifications when Cultured in differentiating medium. Migration and axon-like outgrowth have been successfully guided by means of an interconnected Squares configuration. The surfaces are suitable for controlling the patterning of stem cells and provide a platform for the assessment of the way how different cell arrangements and culture conditions influence cell interactions and cell developmental processes. (c) 2008 Elsevier Ltd. All rights reserved.