Relationship between iron and phosphate in mammalian ferritins.
Relationship between iron and phosphate in mammalian ferritins.
复制标题
哺乳动物铁蛋白中铁和磷酸盐的关系。
DOI：
10.1006/abbi.1993.1308
复制
发表时间：
1993
影响因子：
3.9
通讯作者：
Aust,SD
中科院分区：
文献类型：
--
作者：
deSilva,D;Guo,JH;Aust,SD
已知哺乳动物铁蛋白的核心含有不同数量的磷酸盐和铁。本研究检测了马脾脏、大鼠肝脏和牛肝脏铁蛋白中磷酸盐的变化。磷酸盐的含量与岩心中铁的含量成反比。理论外推表明，在没有磷酸盐的情况下，大约有4400个铁原子可以并入铁蛋白中。铁蛋白与铁和铜蓝蛋白重组，然后与磷酸盐产生的核长时间孵育，在铁与磷酸盐的比率和铁的释放率方面类似于天然铁蛋白。然而，在磷酸盐存在下重组的铁蛋白与天然铁蛋白明显不同。这些数据表明，磷酸盐是哺乳动物铁蛋白核心的一个组成部分，并影响核心的形成和铁从铁蛋白中释放的难易程度。
The core of mammalian ferritin is known to contain varying amounts of phosphate as well as iron. This study examined the variations in phosphate found in ferritins from horse spleen, rat liver, and bovine liver. The amount of phosphate varied inversely with the amount of iron present in the core. Theoretical extrapolation showed that in the absence of phosphate approximately 4400 atoms of iron could be incorporated into ferritin. Reconstitution of ferritin with iron and ceruloplasmin followed by prolonged incubation with phosphate produced cores similar to native ferritin in terms of iron to phosphate ratios and rates of iron release. However, ferritin reconstituted in the presence of phosphate differed markedly from native ferritins. The data suggest that phosphate is an integral part of mammalian ferritin cores and influences both core formation and the ease by which iron is released from ferritin.