IDENTIFICATION OF 2 STRAINS OF CULTURED CANINE RENAL EPITHELIAL-CELLS (MDCK CELLS) WHICH DISPLAY ENTIRELY DIFFERENT PHYSIOLOGICAL-PROPERTIES
IDENTIFICATION OF 2 STRAINS OF CULTURED CANINE RENAL EPITHELIAL-CELLS (MDCK CELLS) WHICH DISPLAY ENTIRELY DIFFERENT PHYSIOLOGICAL-PROPERTIES
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DOI：
10.1113/expphysiol.1981.sp002529
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发表时间：
1981-01-01
影响因子：
--
通讯作者：
SIMMONS, NL
中科院分区：
文献类型：
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作者：
BARKER, G;SIMMONS, NL
在可渗透滤器支持物上生长的Madin-Derby犬肾（MDCK）细胞培养单层显示出体内上皮细胞的许多特征。跨单层电阻，开路p.d. [电位差]、稀释和双离子p.d.由安装在Ussing型室（0.75 cm窗口半径）中的MDCK单层制成。先前报道的跨单层电阻值为100 Ω。cm 2（Cereijido等人，1978; Misfeldt等人，1976年）表明上皮细胞渗漏。这项工作的主要发现是MDCK单层也可以显示出与紧密上皮相似的跨单层电阻值（平均值= 4116 Ω）。cm 2）。这种差异的原因是存在不同的MDCK细胞株。高抗性单层细胞来源于60次连续传代的细胞储备液，而低抗性单层细胞来源于109次连续传代的细胞储备液。跨单层稀释和双离子p.d.的测量。连同Na+示踪剂通量和La 3+渗透通过顶端紧密连接的EM观察，证实了MDCK单层中的高电阻特性的发现，并确认存在显示完全不同的生理特性的2个单独的MDCK细胞株。差异延伸到细胞形态和细胞体积的2株MDCK细胞。
Cultured monolayers of Madin-Derby canine kidney (MDCK) cells grown upon permeable filter supports display many features of in vivo epithelia. Transmonolayer resistance, open-circuit p.d. [potential difference], dilution and bi-ionic p.d. made of MDCK monolayers mounted in Ussing-type chambers (0.75 cm window radius) were made. Previously reported values of transmonolayer resistance of 100 .OMEGA. cm2 (Cereijido et al., 1978; Misfeldt, et al., 1976) indicate a leaky epithelium. The major finding of this work is that MDCK monolayers can also display values of transmonolayer resistance similar to tight epithelia (mean value = 4116 .OMEGA. cm2). The reason for such differences is the existence of separate strains of MDCK cells. High-resistance monolayers originate from cell stocks of 60 serial passages while low-resistance monolayers originate from cell stocks of 109 serial passages. Measurements of transmonolayer dilution, and bi-ionic p.d. together with Na+ tracer fluxes and EM observations of La3+ penetration through the apical tight junctions, substantiate the finding of high-resistance properties in MDCK monolayers and confirm the existence of 2 separate MDCK cell strains displaying entirely different physiological properties. Differences extend to cellular morphology and cellular volume in the 2 strains of MDCK cells.