FUNCTIONAL ORGANIZATION OF DOG SUPERIOR OLIVARY COMPLEX - AN ANATOMICAL AND ELECTROPHYSIOLOGICAL STUDY
FUNCTIONAL ORGANIZATION OF DOG SUPERIOR OLIVARY COMPLEX - AN ANATOMICAL AND ELECTROPHYSIOLOGICAL STUDY
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DOI：
10.1152/jn.1968.31.4.639
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发表时间：
1968-01-01
影响因子：
2.5
通讯作者：
BROWN, PB
中科院分区：
文献类型：
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作者：
GOLDBERG, JM;BROWN, PB
方法解剖四只杂种狗，体重18-30磅。用于追踪从耳蜗复合体到上级橄榄体的纤维。通过切除小脑的外侧部分暴露耳蜗核。用虹膜切除刀造成病变，在某些情况下，通过玻璃吸管抽吸扩大病变。7 - 10天后，用Koenig、Groat和Windle（23）推荐的溶液经心灌注每只动物，最终溶液含3%重铬酸钾（GL Rasmussen，个人交流）。第二天取出脑，置于福尔马林盐水中5至7天，然后修剪。冰冻切片，30 μ m厚，在额状面切割。每隔5个切片用Laidlaw改良的Nauta-Gygax技术染色（5，29）。相邻切片用甲酚紫染色。同样制备的材料从六只猫也是available.The形态的上级橄榄复合体进行了审查，在三个正常的狗的大脑已嵌入火棉胶和切片在30 p。一个大脑被切断在一个正面，另一个在矢状面，和第三个在一个水平面。每个脑的一组切片用甲酚紫染色，第二组用Woelke方法制备，用于显示有髓鞘纤维。还研究了一只正常猫的额状面脑切片。
METHODSAnatomy Four mongrel dogs weighing 18-30 lb. were used to trace fibers from the cochlear complex to the superior olive. The cochlear nucleus was exposed by removal of the lateral part of the cerebellum. Lesions were made with an iridectomy knife and, in some cases, were enlarged by suction through a glass pipette. After 7 to 10 days, each animal was perfused transcardially with the solutions recommended by Koenig, Groat, and Windle (23), the final solution containing 3% potassium dichromate (GL Rasmussen, personal communication). The brain was removed the next day, placed in formolsaline for 5 to 7 days and then trimmed. Frozen sections, 30 p in thickness, were cut in a frontal plane. Every fifth section was stained by the Laidlaw modification of the Nauta-Gygax technique (5, 29). Adjacent sections were stained with cresyl violet. Similarly prepared material from six cats was also available.The morphology of the superior olivary complex was reviewed in three normal dog brains which had been embedded in celloidin and sectioned at 30 p. One of the brains was cut in a frontal, another in a sagittal, and the third in a horizontal plane. A set of sections from each brain was stained with cresyl violet and a second set prepared by Woelke’s method for the demonstration of myelinated fibers. One normal cat brain cut in a frontal plane was also studied.