Higher prevalence of human herpesvirus 8 DNA sequence and specific IgG antibodies in patients with pemphigus in China
Higher prevalence of human herpesvirus 8 DNA sequence and specific IgG antibodies in patients with pemphigus in China
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DOI：
10.1016/j.jaad.2004.10.882
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发表时间：
2005-03-01
影响因子：
13.8
通讯作者：
Chen, HD
中科院分区：
文献类型：
--
作者：
Wang, GQ;Xu, HH;Chen, HD
背景：环境因素，包括病毒感染，可能在天疱疮的发生和/或发展中发挥作用。目的：探讨天疱疮与人类疱疹病毒（human herpesvirus，HHV-8）的关系。共收集了58例天疱疮患者的36例皮损皮肤和13例外周血单个核细胞标本，以及18例正常皮肤和230例健康人的外周血单个核细胞标本，巢式聚合酶链反应检测HHV-8 DNA序列。以HHV-8特异性寡肽为抗原，采用酶联免疫吸附试验检测了29份患者血清和109份健康人血清中的HHV-8特异性IgG抗体。两种HHV-8 DNA序列的流行率皮损区和外周血单个核细胞分别为36.1%和30.8%，天疱疮患者的HHV-8特异性IgG抗体（34.5%）在统计学上高于对照受试者（在两种测定中均< 8%）。结论：HHV-8感染可能是天疱疮发病的一个重要因素。
Background: Environmental factors, including virus infection, may play a role in the onset and/or development of pemphigus. However, it is controversial whether human herpesvirus (HHV)-8 is involved in pathogenesis of pemphigus.Objective: The possible association of pemphigus with HHV-8 was investigated.Methods: A total of 36 lesional skin and 13 peripheral blood mononuclear cell specimens from 58 patients with pemphigus, and 18 normal skin and 230 peripheral blood mononuclear cell specimens from healthy individuals, were tested for HHV-8 DNA sequence by a nested polymerase chain reaction assay. In all, 29 sera from the patients and 109 sera from healthy individuals were tested for HHV-8-specific IgG antibodies by enzyme-linked immunosorbent assays using HHV-8-specific oligopeptides as antigens.Results: Prevalence of both HHV-8 DNA sequence (36.1% and 30.8% in lesional skin and in peripheral blood mononuclear cells, respectively) and HHV-8-specific IgG antibodies (34.5%) for patients with pemphigus was statistically higher than that of control subjects (< 8% in both assays). There was no significant difference in HHV-8 prevalence among different types of pemphigus.Conclusion: HHV-8 infection might be a contributing factor in the development of pemphigus.