Bacteria Inspired Internal Standard SERS Substrate for Quantitative Detection
Bacteria Inspired Internal Standard SERS Substrate for Quantitative Detection
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DOI：
10.1021/acsabm.0c00263
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发表时间：
2021-03-15
影响因子：
4.7
通讯作者：
Zhao, Yanli
中科院分区：
文献类型：
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作者：
Liu, Jiawei;Hong, Zilan;Zhao, Yanli
金属呼吸细菌是环境治理中常用的一种利用其表面合成纳米颗粒来回收金属资源的细菌。然而，通过结合细菌和纳米颗粒的优点来进一步利用生物纳米颗粒仍然是有限的。本文中，生物成因的Au@Ag纳米岛被用作用于定量检测的表面增强拉曼光谱（Sers）基底。具体而言，Au@Ag纳米岛通过表面等离子体共振增强拉曼信号，而生物分子（磷脂、酪氨酸和苯丙氨酸等）通过表面等离子体共振增强拉曼信号。作为内标，消除了不同热点处目标Sers强度的差异。细菌中基因控制的生物分子保证了这种Sers基底的重现性。通过测定罗丹明6 G、孔雀石绿色和尿酸，证明了该分析方法的通用性。这一发现通过一个简单的生物纳米系统解决了Sers分析中的一个普遍问题，这开辟了一条通过使用自然界中的多功能生物来应对科学挑战的途径。
Metal-respiring bacteria are frequently used to recycle metal resources by biosynthesizing nanoparticles on its surface in environment treatment. However, further utilization of biogenetic nanoparticles through combining the advantages of both bacteria and nanoparticles is still limited. Herein, biogenetic Au@Ag nanoislands are utilized as the surface-enhanced Raman spectroscopy (SERS) substrate for quantitative detection. Specifically, Au@Ag nanoislands enhance the Raman signal via surface plasmon resonance, while biomolecules (phospholipid, tyrosine, and phenylalanine, etc.) on bacterium serve as an internal standard to eliminate the discrepancy of the target SERS intensity in different hot spots. Gene-controlled biomolecules in bacteria guarantee the reproducibility of this SERS substrate. The generality of this analytical method is demonstrated by determining rhodamine 6G, malachite green, and uric acid. This discovery solves a pervasive problem in SERS analysis through a simple biogenetic nanosystem, which opens up an avenue to address scientific challenges by using versatile organisms from nature.