Effects of tissue hydration on nanoscale structural morphology and mechanics of individual Type I collagen fibrils in the Brtl mouse model of Osteogenesis Imperfecta.
Effects of tissue hydration on nanoscale structural morphology and mechanics of individual Type I collagen fibrils in the Brtl mouse model of Osteogenesis Imperfecta.
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DOI：
10.1016/j.jsb.2012.09.012
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发表时间：
2012-12
影响因子：
3
通讯作者：
Wallace, Joseph M.
中科院分区：
文献类型：
--
作者：
Kemp, Arika D.;Harding, Chad C.;Cabral, Wayne A.;Marini, Joan C.;Wallace, Joseph M.
I型胶原是哺乳动物中最丰富的蛋白质，并且是许多组织的细胞外基质的重要部分。尽管胶原蛋白的重要性，很少有人知道它在组织中的纳米形态和形态如何与机械功能。本研究探讨了胶原蛋白的纳米结构和机械性能作为疾病的功能在本地水合肌腱。研究了野生型肌腱和来自成骨不全的Brt 1/+小鼠模型的肌腱。使用原子力显微镜（AFM）对水合和脱水条件下的胶原原纤维进行成像和最小化处理。使用AFM是因为能够使生物组织尽可能接近其天然原位条件。该研究证明了水合肌腱中Brt 1/+原纤维形态和力学的表型差异，其在脱水后变得更加引人注目。干肌腱有一个显着的向下移动，在原纤维D-周期间距与湿肌腱的移位。纳米尺度的变化，在干燥样品的形态伴随着显着增加的模量和粘附力和压痕深度降低。一个最小的机械表型存在于水合样品中，可能是由于水掩蔽结构缺陷内的患病原纤维。该研究表明，胶原纳米级形态和力学在Brt 1/+肌腱中受到影响，并且表型可以通过水的存在或不存在来调节。脱水导致需要水的生物样品中的伪影，并且在基于胶原蛋白的组织中以任何长度尺度进行研究时必须考虑该因素，特别是在表征疾病引起的差异时。
Type I collagen is the most abundant protein in mammals, and is a vital part of the extracellular matrix for numerous tissues. Despite collagen’s importance, little is known about its nanoscale morphology in tissues and how morphology relates to mechanical function. This study probes nanoscale structure and mechanical properties of collagen as a function of disease in native hydrated tendons. Wild type tendon and tendon from the Brtl/+ mouse model of Osteogenesis Imperfecta were investigated. An atomic force microscope (AFM) was used to image and indent minimally-processed collagen fibrils in hydrated and dehydrated conditions. AFM was used because of the ability to keep biological tissues as close to their native in situ conditions as possible. The study demonstrated phenotypic difference in Brtl/+ fibril morphology and mechanics in hydrated tendon which became more compelling upon dehydration. Dried tendons had a significant downward shift in fibril D-periodic spacing versus a shift up in wet tendons. Nanoscale changes in morphology in dry samples were accompanied by significant increases in modulus and adhesion force and decreased indentation depth. A minimal mechanical phenotype existed in hydrated samples, possibly due to water masking structural defects within the diseased fibrils. This study demonstrates that collagen nanoscale morphology and mechanics are impacted in Brtl/+ tendons, and that the phenotype can be modulated by the presence or absence of water. Dehydration causes artifacts in biological samples which require water and this factor must be considered for studies at any length scale in collagen-based tissues, especially when characterizing disease-induced differences.
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影响因子：
56.9
作者：
BELLA, J;EATON, M;BERMAN, HM
通讯作者：
BERMAN, HM
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作者：
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影响因子：
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作者：
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