Genetic mapping of two pairs of linked ribosomal protein genes in Saccharomyces cerevisiae.
Genetic mapping of two pairs of linked ribosomal protein genes in Saccharomyces cerevisiae.
复制标题
酿酒酵母中两对连锁核糖体蛋白基因的遗传图谱。
DOI：
10.1007/bf00384605
复制
发表时间：
1987
期刊：
影响因子：
2.5
通讯作者：
Pearson,NJ
中科院分区：
文献类型：
--
作者：
Papciak,SM;Pearson,NJ
We have used the 2μmapping method described by Falco and Botstein (1983) and tetrad analysis to map four ribosomal protein genes (two linked pairs) inS. cerevisiae. One pair (rp28–rp55 copy 1) is on chromosome XV, 14 cM proximal toARG8. The other pair (rp55–rp28 copy 2) is 19 cM from the centromere on the left arm of chromosome XIV. To map copy 1 we used theE. coli β-galactosidase gene rather than a yeast gene to mark the ribosomal protein chromosomal locus. This provided a more sensitive color screening assay for chromosome loss in the 2μmethod. It also removed the restriction that the mapping tester strains must be mutant for the plasmid marker.
