HOST SPECIFICITY OF DNA PRODUCED BY ESCHERICHIA-COLI .1. HOST CONTROLLED MODIFICATION OF BACTERIOPHAGE LAMBDA
HOST SPECIFICITY OF DNA PRODUCED BY ESCHERICHIA-COLI .1. HOST CONTROLLED MODIFICATION OF BACTERIOPHAGE LAMBDA
复制标题
DOI：
10.1016/s0022-2836(62)80058-8
复制
发表时间：
1962-01-01
影响因子：
5.6
通讯作者：
DUSSOIX, D
中科院分区：
文献类型：
--
作者：
ARBER, W;DUSSOIX, D
λ噬菌体颗粒携带由产生它们的细菌菌株决定的“宿主特异性”。在感染不同的细菌宿主时，（1）噬菌体DNA可以根据这种特异性被接受或排斥，（2）如果被接受，噬菌体繁殖并产生子代噬菌体。亲本噬菌体DNA分子以保守或半保守形式转移到其上的那些子代也接受亲本噬菌体宿主特异性。所有只含有新合成DNA的后代只接受新细菌宿主的特异性。因此，噬菌体DNA具有宿主特异性。噬菌体P1，以原噬菌体或营养噬菌体的形式存在于细菌细胞中，赋予在同一细胞中增殖的A DNA宿主特异性，其特异性超过宿主本身所决定的特异性。这种P1诱导的特异性可以同样好地施加到复制和非复制K DNA上。
Lambda bacteriophage particles carry a "host specificity" determined by the bacterial strains on which they were produced. Upon infection of a different bacterial host (1) the phage DNA may be either accepted or rejected on the basis of this specificity, (2) if accepted, the phage multiplies and progeny phage are produced. Those progeny to which the parental phage DNA molecule is transferred, in either conserved or semi-conserved form, also receive the parental phage host specificity. All progeny containing only newly synthesized DNA receive only the specificity of the new bacterial host. It is concluded that host specificity is carried on the bacteriophage DNA. Phage P1, present in a bacterial cell as either prophage or vegetative phage, imparts to A DNA multiplying in the same cell a host specificity over and above that determined by the host itself. Such P1- induced specificity can be impressed equally well onto replicating and non-replicating K DNA.