Characterization of emY162 encoding an immunogenic protein cloned from an adult worm-specific cDNA library of Echinococcus multilocularis
Characterization of emY162 encoding an immunogenic protein cloned from an adult worm-specific cDNA library of Echinococcus multilocularis
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DOI：
10.1016/j.bbagen.2007.08.020
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发表时间：
2008-01-01
影响因子：
3
通讯作者：
Yagi, Kinpei
中科院分区：
文献类型：
--
作者：
Katoh, Yoshinobu;Kouguchi, Hirokazu;Yagi, Kinpei
以多房棘球绦虫蠕虫mRNA为基础构建cDNA文库。从该cDNA文库中分离到一个cDNA克隆emY 162。emY 162 cDNA的推定蛋白质由153个氨基酸组成，预测分子量为17.0 kDa。EMY 162的氨基酸序列预测为疏水性N-末端保守分泌信号，和疏水性C-末端编码跨膜结构域或糖基-磷酸肌醇膜锚，并且具有单个III型纤连蛋白样结构域。此外，还对E.多房虫基因组DNA由3个外显子和2个内含子组成，emY 162在所有4个阶段（原绦虫、培养的后绦虫、未成熟蠕虫和成熟蠕虫）中表达。此外，检测了对重组EMY 162的免疫力，所述重组EMY 162包含EMY 162蛋白上的纤连蛋白III型样结构域。用E.多房的在Western印迹中检测到强IgG免疫应答。(C)2007 Elsevier B. V.保留所有权利。
A cDNA library based on mRNA from adult worms of Echinococcus multilocularis was constructed. One cDNA clone, emY162, was isolated from this cDNA library. The putative protein from emY162 cDNA consists of 153 amino acids and has a predicted molecular weight of 17.0 kDa. The amino acid sequences of EMY162 are predicted to be a hydrophobic N-terminus conserving a secretory signal, and a hydrophobic C-terminus encoding a transmembrane domain or glycosyl-phosphatylinositol membrane anchor, and to have single fibronectin type III-like domain. In addition, it was shown that the emY162 gene (1738 bp) in the E. multilocularis genome DNA consists of three exons and two introns, and that emY162 is expressed in all four stages (protoscoleces, cultured metacestodes, immature adult worms and mature adult worms). Moreover, immunity to recombinant EMY162, which comprises the fibronectin type III-like domain on the EMY162 protein, was examined. Immune responses to the recombinant EMY162 were studied by using serum from dogs infected with E. multilocularis. Strong IgG immune responses were detected in Western blots. (C) 2007 Elsevier B.V. All rights reserved.