Enteral nutrient intake level determines intestinal protein synthesis and accretion rates in neonatal pigs.
Enteral nutrient intake level determines intestinal protein synthesis and accretion rates in neonatal pigs.
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DOI：
10.1152/ajpgi.2000.279.2.g288
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发表时间：
2000-08
期刊：
影响因子：
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通讯作者：
B. Stoll;X. Chang;M. Fan;P. Reeds;D. Burrin
中科院分区：
文献类型：
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作者：
B. Stoll;X. Chang;M. Fan;P. Reeds;D. Burrin
我们的目的是确定增加新生儿小肠蛋白质增积率（PAR）所需的最低肠内摄入水平。7日龄仔猪饲喂等量日基础饲粮，并按不同比例（0、10%、20%、40%、60%、80%和100%）进行肠内饲喂。7 d后，仔猪静脉注射[(2)H(3)]亮氨酸6 H，测定空肠近端（PJ）和远端（DJ）以及回肠近端（PI）和远端（DI）的蛋白质合成率（FSR）。当肠内摄取量为0 ~ 60%时，空肠FSR从45%/d增加到130%/d，而回肠FSR对肠内摄取量的敏感性较低。在0%肠内摄入量时，PAR在PJ、DJ和PI中显著为负（范围为-70至-43 mg/天），在DI中呈阳性（范围为49 mg/天），而在20%肠内摄入量时，肠道蛋白质出现平衡。在100%肠内摄入时，尽管蛋白质转化率比PJ低50%，但DI组的PAR最高。我们得出结论，肠道蛋白质净损失在0%肠内摄入量，蛋白质平衡在20%肠内摄入量，最大肠道蛋白质增加60%肠内摄入量。
Our objective was to determine the minimum enteral intake level necessary to increase the protein accretion rate (PAR) in the neonatal small intestine. Seven-day-old piglets received an equal total daily intake of an elemental diet, with different proportions given enterally (0, 10%, 20%, 40%, 60%, 80%, and 100%). After 7 days, piglets were infused intravenously with [(2)H(3)]leucine for 6 h, and the fractional protein synthesis rate (FSR) was measured in the proximal (PJ) and distal jejunum (DJ) and the proximal (PI) and distal ileum (DI). The jejunal FSR increased from 45%/day to 130%/day between 0 and 60% enteral intake, whereas the FSR in the ileum was less sensitive to enteral intake level. At 0% enteral intake, PAR was significantly negative in the PJ, DJ, and PI (range -70 to -43 mg/day) and positive in the DI (49 mg/day), whereas intestinal protein balance occurred at 20% enteral intake. At 100% enteral intake, the PAR was greatest in the DI, even though the rates of protein turnover were 50% lower than in the PJ. We conclude that there is net intestinal protein loss at 0% enteral intake, protein balance at 20% enteral intake, and maximal intestinal protein accretion at 60% enteral intake.