Synthesis, characterization of calcium phosphates/polyurethane composites for weight-bearing implants.
Synthesis, characterization of calcium phosphates/polyurethane composites for weight-bearing implants.
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DOI：
10.1002/jbm.b.31917
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发表时间：
2012
期刊：
影响因子：
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通讯作者：
T. Yoshii;Jerald E. Dumas;A. Okawa;D. Spengler;S. Guelcher
中科院分区：
文献类型：
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作者：
T. Yoshii;Jerald E. Dumas;A. Okawa;D. Spengler;S. Guelcher
磷酸钙(CaP)/聚合物复合材料作为一种替代性的骨移植材料已被研究。本研究以羟基磷灰石(HA)和β-磷酸三钙为原料，合成了赖氨酸-三异氰酸酯基聚氨酯(PUR)复合材料，以降低壳体的脆性，提高聚合物的生物活性。研究了HA/PUR和TCP/PUR复合材料的力学性能和体外细胞响应。将复合材料植入大鼠股骨缺损处，通过X射线、微电脑断层扫描(μCT)和组织学切片评价体内生物活性。在生物力学测试中，PUR改善了帽子的机械性能，从而使其潜在地适合于负重应用。体外细胞培养研究表明，β/PUR复合材料具有良好的生物相容性，与HA相比，CAP/PUR复合材料增强了细胞的活力和增殖能力。CaP/PUR复合材料也支持成骨细胞在材料上的分化。当植入大鼠股骨缺损处时，CaP/PUR复合材料具有生物相容性和骨传导性，X射线、μCT图像和组织切片证明没有不良炎症反应。此外，组织学检查显示有细胞浸润和对位重塑的证据。这些结果表明，CaP/PUR复合材料有可能成为负重骨科植入物的潜在生物材料。
Calcium phosphate (CaP)/polymer composites have been studied as an alternative graft material for the treatment of bone defects. In this study, lysine-triisocyanate-based polyurethane (PUR) composites were synthesized from both hydroxyapatite (HA) and β-tricalcium phosphate (TCP) to reduce the brittleness of CaP and increase the bioactivity of the polymer. The mechanical properties and in vitro cellular response were investigated for both HA/PUR and TCP/PUR composites. The composites were implanted in femoral defects in rats, and in vivo bioactivity was evaluated by X-rays, micro-computed tomography (μCT), and histological sections. In biomechanical testing, PUR improved the mechanical properties of the CaP, thus rendering it potentially suitable for weight-bearing applications. In vitro cell culture studies showed that CaP/PUR composites are biocompatible, with β-TCP enhancing the cell viability and proliferation relative to HA. CaP/PUR composites also supported the differentiation of osteoblastic cells on the materials. When implanted in rat femoral defects, the CaP/PUR composites were biocompatible and osteoconductive with no adverse inflammatory response, as evidenced by X-rays, μCT images, and histological sections. Additionally, a histological examination showed evidence of cellular infiltration and appositional remodeling. These results suggest that CaP/PUR composites could be potentially useful biomaterials for weight-bearing orthopaedic implants.