Goigin tethers define subpopulations of COPI vesicles
Goigin tethers define subpopulations of COPI vesicles
复制标题
DOI：
10.1126/science.1108061
复制
发表时间：
2005-02-18
期刊：
影响因子：
56.9
通讯作者：
Warren, G
中科院分区：
文献类型：
--
作者：
Malsam, J;Satoh, A;Warren, G
卷曲螺旋蛋白的高尔基体家族已牵连在高尔基体内运输通过拴系外壳蛋白复合物I（COPI）囊泡。p115-golgin系链是最好的研究，在这里，我们的特点golgin-84-CASP系链。由这种系绳结合的囊泡与由p115-golgin系绳结合的囊泡明显不同，因为它们缺乏推定的货物受体的p24家族的成员，并且含有酶而不是顺行货物。显微注射高尔基体-84或CASP也抑制高尔基体酶运输到内质网，进一步牵连这条系绳逆行运输。这些和其他高尔基体可以调节高尔基体堆栈内的流动模式。
Coiled-coil proteins of the golgin family have been implicated in intra-Golgi transport through tethering coat protein complex I (COPI) vesicles. The p115-golgin tether is the best studied, and here we characterize the golgin-84-CASP tether. The vesicles bound by this tether were strikingly different from those bound by the p115-golgin tether in that they lacked members of the p24 family of putative cargo receptors and contained enzymes instead of anterograde cargo. Microinjected golgin-84 or CASP also inhibited Golgi-enzyme transport to the endoplasmic reticulum, further implicating this tether in retrograde transport. These and other golgins may modulate the flow patterns within the Golgi stack.