Serological Prevalence of Hepatitis E Virus in Domestic Animals and Diversity of Genotype 4 Hepatitis E Virus in China
Serological Prevalence of Hepatitis E Virus in Domestic Animals and Diversity of Genotype 4 Hepatitis E Virus in China
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DOI：
10.1089/vbz.2009.0168
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发表时间：
2010-10-01
影响因子：
2.1
通讯作者：
Wang, Youchun
中科院分区：
文献类型：
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作者：
Geng, Yansheng;Wang, Chuanbin;Wang, Youchun
已证实猪是戊型肝炎病毒（HEV）某些基因型的宿主，其他非人类物种也可能感染该病毒。为了评估中国家畜HEV感染的流行程度，从全国26个省收集了3579份血清样本，包括1967份猪血清、700份山羊血清和912份牛血清，并使用酶免疫测定法检测了HEV抗体和抗原。结果表明，82.2%的猪血清、10.4%的牛血清和28.2%的山羊血清分别呈hev抗体阳性。动物中抗hev抗体的流行率因省而异，猪为10.9%至100%，山羊为0%至48%，牛为0%至92.9%。在该研究中，约1.9%的猪、1.6%的山羊和0.8%的牛检测出戊型肝炎病毒抗原阳性。一些样本，包括所有HEV抗原阳性样本，使用逆转录聚合酶链反应检测HEV特异性RNA。15个猪样本，但没有山羊或牛样本，被发现戊型肝炎病毒RNA阳性。序列分析和系统发育分析将所有分离株归为HEV基因4型，并将其进一步划分为4个亚群。这项研究表明，在中国，HEV感染在家畜，特别是猪中广泛存在。中国猪感染的HEV基因型为基因4型。然而，这些分离物表现出相当大的遗传多样性。
Pigs have been confirmed to be reservoirs of some genotypes of hepatitis E virus (HEV), and other nonhuman species are also likely infected with the virus. To assess the prevalence of HEV infection in domestic animals in China, 3579 serum samples, including 1967 swine, 700 goat, and 912 cattle sera, were collected from 26 provinces across the country and tested for HEV antibodies and antigen using enzyme immunoassays. The results showed that 82.2% of the swine samples, but only 10.4% and 28.2% of cattle and goat sera, were anti-HEV positive respectively. The prevalence of anti-HEV antibody in animals varied from province to province, ranging from 10.9% to 100% in pigs, 0% to 48% in goats, and 0% to 92.9% in cattle. About 1.9% of pigs, 1.6% of goats, and 0.8% of cattle tested in the study were positive for HEV antigen. Some samples, including all HEV antigen-positive samples, were tested for HEV-specific RNA using reverse transcription polymerase chain reaction. Fifteen swine samples, but none from the goats or cattle, were found to be HEV RNA positive. Sequence and phylogenetic analyses classified all the swine HEV isolates into HEV genotype 4, which was further divided into four subgroups. This study demonstrated that HEV infection is widespread in domestic animals, particularly pigs, in China. The HEV genotype infecting pigs in China was genotype 4. However, the isolates displayed considerable genetic diversity.