Sheath proteins: synthesis, secretion, degradation and fate in forming enamel.
Sheath proteins: synthesis, secretion, degradation and fate in forming enamel.
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鞘蛋白：合成、分泌、降解和形成牙釉质的命运。
DOI：
10.1111/j.1600-0722.1998.tb02191.x
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发表时间：
1998
影响因子：
1.9
通讯作者：
Osamu Takahashi
中科院分区：
文献类型：
--
作者：
Takashi Uchida;C. Murakami;K. Wakida;Naofumi Dohi;Yasuko Iwai;J. P. Simmer;M. Fukae;T. Satoda;Osamu Takahashi
我们研究了成釉蛋白和鞘蛋白，最近克隆的釉基质蛋白从大鼠和猪，在形成釉质免疫细胞化学和免疫化学，使用区域特异性抗体的表达。从大鼠和猪中获得的结果基本相同。识别N-末端区域的抗体染色的分泌性成釉细胞的分泌机制和釉基质的整个厚度，特别是釉棒的周边区域。在65或70 kDa附近和低于20 kDa处观察到免疫染色蛋白条带。C-末端特异性抗体染色的成釉细胞和未成熟的釉质分泌部位附近的分泌机制。免疫染色的蛋白质带被发现范围从25至70 kDa。抗体识别的蛋白质中的一个区域之前的C-末端区域染色的高尔基体的顺式侧，但不是釉基质。观察到约55 kDa的免疫染色蛋白条带。这些结果表明，成釉蛋白和鞘蛋白的翻译后和分泌后修饰是彼此相似的，并进一步表明，其切割的N端多肽集中在棱柱鞘。我们认为鞘蛋白和成釉蛋白在成釉过程中具有相同的作用，应被归类为鞘蛋白。
We investigated expression of ameloblastin and sheathlin, recently cloned enamel matrix proteins from the rat and pig, in forming enamel immunocytochemically and immunochemically, using region-specific antibodies. The results obtained from the rat and pig were essentially the same. Antibodies which recognize the N-terminal region stained the secretory machinery of the secretory ameloblast and the entire thickness of the enamel matrix, especially the peripheral region of the enamel rod. Immunostained protein bands were observed near 65 or 70 kDa and below 20 kDa. C-terminal-specific antibodies stained the secretory machinery of the ameloblast and the immature enamel adjacent to the secretion sites. Immunostained protein bands were found ranging from 25 to 70 kDa. Antibodies which recognize a region in the protein just prior to the C-terminal region stained the cis-side of the Golgi apparatus but not the enamel matrix. Immunostained protein bands were observed of about 55 kDa. These results suggest that post-translational and post-secretory modifications of ameloblastin and sheathlin are similar to each other, and further showed that their cleaved N-terminal polypeptides concentrate in the prism sheath. We propose that sheathlin and ameloblastin share the same role in amelogenesis and should be classified as sheath proteins.