Myocardial homing of nonmobilized peripheral-blood CD34+ cells after intracoronary injection
Myocardial homing of nonmobilized peripheral-blood CD34+ cells after intracoronary injection
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DOI：
10.1634/stemcells.2005-0201
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发表时间：
2006-02-01
期刊：
影响因子：
5.2
通讯作者：
Goldman, Serge
中科院分区：
文献类型：
--
作者：
Blocklet, Didier;Toungouz, Michel;Goldman, Serge
粒细胞集落刺激因子用于从血液中分离自体造血干细胞可能有利于急性心肌梗死（AMI）后植入患者的再狭窄。因此，我们在六名AMI患者中测试了无需动员的外周血CD 34（+）细胞的分离。经大体积细胞分离和阳性CD 34（+）细胞筛选后，获得360万至2760万个CD 34（+）细胞。我们进行了冠状动脉内植入这些细胞，并记录没有再狭窄或心律失常。我们使用正电子发射断层扫描（PET）来评估心肌标记的CD 34（+）细胞归巢，植入后1小时占注射细胞的5.5%。总之，大量的CD 34（+）细胞，在以前的研究报告的范围内，可以从未动员的外周血中获得。PET与[F-18]-氟脱氧葡萄糖细胞标记是一种有效的成像方法归巢评估。
Granulocyte-colony-stimulating factor administered for autologous hematopoietic stem cell isolation from blood may favor restenosis in patients implanted after acute myocardial infarction (AMI). We therefore tested the isolation of peripheral-blood CD34(+) cells without mobilization in six patients with AMI. After large-volume cytapheresis and positive CD34(+) cell selection, 3.6 to 27.6 million CD34(+) cells were obtained. We performed intracoronary implantation of these cells and recorded no restenosis or arrhythmia. We used positron emission tomography (PET) to assess myocardial-labeled CD34(+) cell homing, which accounted for 5.5% of injected cells I hour after implantation. In conclusion, large amounts of CD34(+) cells, in the range reported in previous studies, can be obtained from nonmobilized peripheral blood. PET with [F-18]-fluorodeoxyglucose cell labeling is an efficient imaging method for homing assessment.