Dual-functional polyetheretherketone surface modification for regulating immunity and bone metabolism
Dual-functional polyetheretherketone surface modification for regulating immunity and bone metabolism
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DOI：
10.1016/j.cej.2021.130806
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发表时间：
2021-06-19
影响因子：
15.1
通讯作者：
Yue, Bing
中科院分区：
文献类型：
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作者：
Huo, Shicheng;Wang, Fan;Yue, Bing
近年来，聚醚醚酮（PEEK）作为骨科植入材料越来越受到关注。然而，PEEK表面引起的不良炎症和免疫反应导致骨整合较差，影响其临床应用。为了改善骨整合并提高PEEK植入物的成功率，可以将表面生物功能化以具有免疫调节作用。在此，A-485，一个有效的和选择性的p300/CBP的催化抑制剂，装载到介孔生物活性玻璃，已固定在PEEK表面，和生物功能化的PEEK植入物的免疫调节能力和对骨整合的影响进行了评价。在体外，生物功能化的PEEK表面不仅抑制巨噬细胞的急性炎症反应，而且还抑制骨吸收和促进成骨，表明它在维持正常骨代谢中起着关键而复杂的作用。此外，生物功能化表面诱导较少的纤维囊形成，并在体内表现出优越的上级骨整合特性。负载A485的MBG生物功能化PEEK表面减弱了巨噬细胞介导的不适当激活的免疫反应，随后抑制破骨细胞生成，促进骨生成并产生优异的上级骨整合。因此，这些表面生物功能化的PEEK植入物，调节免疫和骨代谢，显示出作为骨科植入物的临床应用的强大潜力。
Polyetheretherketone (PEEK) has gained increased attention as an orthopedic implant material in recent years. However, the adverse inflammatory and immunological reactions elicited by the PEEK surface result in inferior osteointegration and affect its clinical utility. To improve osseointegration and enhance the success rate of PEEK implants, the surface can be biofunctionalized to be immunomodulatory. Herein, A-485, a potent and selective catalytic inhibitor of p300/CBP, was loaded into mesoporous bioactive glass that had been immobilized on the PEEK surface, and the immunomodulatory capability and effects of the biofunctionalized PEEK implants on osteointegration were evaluated. In vitro, the biofunctionalized PEEK surface not only suppressed the acute inflammatory response of macrophages, but also inhibited bone resorption and promoted osteogenesis, indicating that it plays critical and complex roles in maintaining normal bone metabolism. Moreover, the biofunctionalized surface induced less fibrous capsule formation and exhibited superior osseointegration properties in vivo. The A485-loaded MBG-biofunctionalized PEEK surface attenuated inappropriately activated immune responses mediated by macrophages and subsequently inhibited osteoclastogenesis, promoting osteogenesis and yielding superior osseointegration. Therefore, these surface-biofunctionalized PEEK implants, which regulate both immunity and bone metabolism, demonstrate strong potential for clinical application as orthopedic implants.