Increased circulating platelet-leucocyte complexes and platelet activation in patients with antiphospholipid syndrome, systemic lupus erythematosus and rheumatoid arthritis
Increased circulating platelet-leucocyte complexes and platelet activation in patients with antiphospholipid syndrome, systemic lupus erythematosus and rheumatoid arthritis
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DOI：
10.1046/j.1365-2141.2001.03101.x
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发表时间：
2001-11-01
影响因子：
6.5
通讯作者：
Machin, SJ
中科院分区：
文献类型：
--
作者：
Joseph, JE;Harrison, P;Machin, SJ
血小板活化可能在与抗磷脂抗体（阿帕）相关的血栓形成风险增加中起致病作用。在这项研究中，在体内血小板活化水平测定20例原发性抗磷脂综合征（PAPS）和30例系统性红斑狼疮（SLE）患者（其中14例继发性APS）使用敏感的流式细胞术。还测定了可溶性P-选择素水平。PAPS患者血小板CD 63表达明显高于正常对照组（P = 0.007），也高于SLE伴和不伴继发性APS患者（P = 0.03和P = 0.002）。PAPS患者的PAC-1结合率显著高于对照组（P = 0.007）和无APS的SLE患者（P = 0.015）。SLE患者的血小板-白细胞复合物显著高于PAPS和对照组，RAPS患者的血小板-单核细胞复合物显著高于对照组（10例无阿帕的类风湿关节炎（RA）患者的血小板-白细胞复合物也显著高于对照组）。PAPS组和SLE组可溶性P-选择素水平均显著高于对照组。血小板CD 62 p表达。PAPS或SLE患者的膜联蛋白V结合率和血小板微粒数没有增加。我们的结论是，有证据表明PAPS和SLE的血小板活化增加，这是很重要的注意，因为它可能有潜在的治疗意义，在这些患者中使用抗血小板药物。
It is possible that platelet activation may play a pathogenic role in the increased risk of thrombosis associated with antiphospholipid antibodies (APA). In this study, levels of in vivo platelet activation were measured in 20 patients with primary antiphospholipid syndrome (PAPS) and 30 systemic lupus erythematosus (SLE) patients (14 of whom had secondary APS) using sensitive flow cytometry. Soluble P-selectin levels were also assayed. Platelet CD63 expression was significantly higher in PAPS than normal controls (P = 0.007), as well as SLE patients with and without secondary APS (P = 0.03 and P = 0.002 respectively). PAC-1 binding was significantly higher in PAPS than the control group (P = 0.007) and SLE patients without APS (P = 0.015). Platelet-leucocyte complexes were significantly higher in SLE patients than both PAPS and the control group, and platelet-monocyte complexes were significantly increased in RAPS compared with the control group, (Platelet-leucocyte complexes were also significantly higher than controls in 10 rheumatoid arthritis (RA) patients without APA). Soluble P-selectin levels were significantly higher in PAPS and SLE patients than the control group. Platelet CD62p expression. annexin V binding and platelet microparticle numbers were not increased in PAPS or SLE patients. We conclude that there is evidence of increased platelet activation in PAPS and SLE, and this is important to note as it may have potential therapeutic implications with respect to use of antiplatelet agents in these patients.