SWITCHING AT THE CELLULAR-LEVEL IN THE WHITE-OPAQUE TRANSITION OF CANDIDA-ALBICANS
SWITCHING AT THE CELLULAR-LEVEL IN THE WHITE-OPAQUE TRANSITION OF CANDIDA-ALBICANS
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DOI：
10.1099/00221287-136-10-1925
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发表时间：
1990-10-01
期刊：
影响因子：
--
通讯作者：
SOLL, DR
中科院分区：
文献类型：
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作者：
BERGEN, MS;VOSS, E;SOLL, DR
白色念珠菌菌株WO-1中的“白色-不透明转变”提供了一个独特的系统，用于分析细胞水平上的高频转换，因为白色和不透明相的出芽表型存在差异。将单个白色和不透明细胞置于琼脂上，并监测细胞分裂、小菌落发生和转换至交替期的动力学。证明了在24 ℃下，不透明细胞可以直接转变为白色细胞，但白色细胞在转变为不透明细胞时首先产生细长的假菌丝形前体。在任一阶段的细胞可以在交替阶段产生子细胞，然后立即恢复到原始阶段的后续子细胞的发生。通过开发一个在细胞水平上转换的数学模型，该模型从转换候选细胞库中减去母细胞和转换的子细胞，计算出不透明细胞在任何一代中产生白色子细胞的概率为1.0 × 10 - 6。10-1，并且计算出白色细胞在任何单代中产生不透明子细胞的概率为1.7 × 10 - 1。10-5 24 ℃在营养琼脂上培养。在24 ℃的营养琼脂上，不透明细胞生成白色子细胞之前的平均代数计算为3.4，白色细胞形成不透明细胞之前的平均代数计算为15.8。最后，在细胞水平上分析了高温诱导的不透明到白色转变，并证明涉及在原始不透明母细胞上频繁形成白色子细胞的双极，以及在某些情况下的中间表型。
The ''white-opaque transition'' in Candida albicans strain WO-1 provides a unique system for analysing high-frequency switching at the cellular level because of the difference in the budding phenotypes of the white and opaque phases. Single white and opaque cells were placed on agar and monitored for the dynamics of cell division, microcolony genesis and switching to the alternative phase. It is demonstrated that at 24.degree.C, opaque cells can switch directly to white cells but white cells first generate an elongate, pseudohyphal-shaped precursor in the transition to an opaque cell. Cells in either phase can generate a daughter cell in the alternative phase, then revert immediately to the genesis of subsequent daughter cells in the original phase. By developing a mathematical model for switching at the cellular level which subtracts mother cells and switched daughter cells from the pool of switching candidates, the probability for an opaque cell to generate a white daughter cell in any single generation was calculated to be 1.0 .times. 10-1, and the probability for a white cell to generate an opaque daughter cell in any single generation was calculated to be 1.7 .times. 10-5 at 24.degree.C on nutrient agar. The mean number of generations before an opaque cell generated a white daughter cell was calculated to be 3.4 and the mean number before a white cell formed an opaque cell was calculated to be 15.8 at 24.degree.C on nutrient agar. Finally, high-temperature induction of the opaque to white transition was analysed at the cellular level and demonstrated to involve frequent bipolar formation of white daughter cells on the original opaque mother cell, and in some cases intermediate phenotypes.