GLUCOCORTICOIDS REGULATE PROOPIOMELANOCORTIN GENE-EXPRESSION INVIVO AT THE LEVELS OF TRANSCRIPTION AND SECRETION
GLUCOCORTICOIDS REGULATE PROOPIOMELANOCORTIN GENE-EXPRESSION INVIVO AT THE LEVELS OF TRANSCRIPTION AND SECRETION
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DOI：
10.1073/pnas.80.22.6982
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发表时间：
1983-01-01
期刊：
影响因子：
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通讯作者：
HERBERT, E
中科院分区：
文献类型：
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作者：
BIRNBERG, NC;LISSITZKY, JC;HERBERT, E
肾上腺切除后，大鼠血浆ACTH/内啡肽水平在前4小时急剧上升，而垂体肽水平急剧下降。肾上腺切除术后8小时，血浆水平再次接近对照值，但随后在接下来的8天内持续升高。前阿黑皮素（POMC）mRNA水平在垂体前叶（定量与克隆POMC c[互补]DNA杂交）增加2倍，在第一个24小时，达到15- 20倍的控制水平后18天肾上腺切除术。肾上腺切除术后8天给予大鼠地塞米松，上述事件逆转。血浆ACTH福尔斯在2小时内下降到对照水平，而垂体前叶POMC mRNA需要5天的治疗才能恢复到对照水平。POMC mRNA在神经中间叶和下丘脑的水平没有改变任何治疗。肾上腺切除术增加了POMC基因在垂体前叶的转录约20倍，并在手术后1小时内一半的生长激素基因的转录。肾上腺切除术后立即给予地塞米松抑制POMC基因转录的增加并增加生长激素基因的转录。神经中间叶中POMC基因的转录不被这些治疗中的任一种改变。
After adrenalectomy, the plasma levels of ACTH/endorphin peptides in rats rise dramatically in the first 4 h while pituitary peptide levels fall sharply. Eight h after adrenalectomy, plasma levels are near control values again but they then increase continuously over the next 8 days. Proopiomelanocortin (POMC) mRNA levels in the anterior pituitary (quantitated by hybridization with cloned POMC c[complementary]DNA) increase 2-fold in the first 24 h, reaching 15- to 20-fold the control level 18 days after adrenalectomy. When dexamethasone is administered to rats 8 days after adrenalectomy, the above events are reversed. Plasma ACTH falls to control levels within 2 h whereas anterior pituitary POMC mRNA requires 5 days of treatment for return to control levels. The levels of POMC mRNA in the neurointermediate lobe and the hypothalamus are not altered by either treatment. Adrenalectomy increases transcription of the POMC gene in the anterior pituitary approximately 20-fold and halves transcription of the growth hormone gene within 1 h of operation. Administration of dexamethasone immediately after adrenalectomy suppresses the increase in transcription of the POMC gene and increases the transcription of the growth hormone gene. Transcription of the POMC gene(s) in the neurointermediate lobe is not altered by either of these treatments.