DNA-damage-inducible (din) loci are transcriptionally activated in competent Bacillus subtilis.
DNA-damage-inducible (din) loci are transcriptionally activated in competent Bacillus subtilis.
复制标题
DNA 损伤诱导 (din) 位点在感受态枯草芽孢杆菌中被转录激活。
DOI：
10.1073/pnas.82.18.6201
复制
发表时间：
1985
影响因子：
11.1
通讯作者：
Yasbin,RE
中科院分区：
文献类型：
--
作者：
Love,PE;Lyle,MJ;Yasbin,RE
通过转座诱变在枯草芽孢杆菌中产生DNA损伤诱导型（din）操纵子融合体。这些YB 886（din：：Tn 917-lacZ）融合分离株在暴露于丝裂霉素C、UV辐射或甲磺酸乙酯时产生增加的β-半乳糖苷酶，表明lacZ结构基因已插入到由多种DNA损伤剂诱导的宿主转录单位中。其中一个融合菌株是DNA修复缺陷型，表型上类似于B的紫外线敏感突变体。枯草杆菌。β-半乳糖苷酶的诱导也发生在每个融合菌株的主管亚群中，独立于DNA损伤剂的暴露。这两个DNA损伤诱导和能力诱导的β-半乳糖苷酶表达的组件被废除的recE 4突变，这是一个类似于SOS（SOS）的诱导，但不干扰发展的主管状态。结果表明，基因表达在B内的特定位点被刺激。通过DNA损伤剂和感受态的发展，枯草杆菌染色体的反应，这种反应是在枯草杆菌调节系统的控制下。此外，他们证明，在分子水平上，诱导和发展的能力是相互关联的细胞事件。
DNA damage-inducible (din) operon fusions were generated in Bacillus subtilis by transpositional mutagenesis. These YB886(din::Tn917-lacZ) fusion isolates produced increased beta-galactosidase when exposed to mitomycin C, UV radiation, or ethyl methanesulfonate, indicating that the lacZ structural gene had inserted into host transcriptional units that are induced by a variety of DNA-damaging agents. One of the fusion strains was DNA-repair deficient and phenotypically resembled a UV-sensitive mutant of B. subtilis. Induction of beta-galactosidase also occurred in the competent subpopulation of each of the din fusion strains, independent of exposure to DNA-damaging agents. Both the DNA-damage-inducible and competence-inducible components of beta-galactosidase expression were abolished by the recE4 mutation, which inhibitS SOS-like (SOB) induction but does not interfere with the development of the competent state. The results indicate that gene expression is stimulated at specific loci within the B. subtilis chromosome both by DNA-damaging agents and by the development of competence and that this response is under the control of the SOB regulatory system. Furthermore, they demonstrate that at the molecular level SOB induction and the development of competence are interrelated cellular events.